The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1193, Japan.
Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1193, Japan.
Sci Rep. 2021 Feb 3;11(1):2951. doi: 10.1038/s41598-021-82598-2.
Milk small extracellular vesicles (sEV) contain proteins that provide potential information of host physiology and immunology. Bovine leukemia virus (BLV) is an oncogenic virus that causes progressive B-cell lymphosarcoma in cattle. In this study, we aimed to explore the proteomic profile of milk sEV from BLV-infected cattle compared with those from uninfected cattle. Milk sEV were isolated from three BLV-infected and three uninfected cattle. Proteomic analysis was performed by using a comprehensive nanoLC-MS/MS method. Furthermore, gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were used to evaluate the candidates for uniquely or differentially expressed proteins in milk sEV from BLV-infected cattle. Proteomic analysis revealed a total of 1330 common proteins in milk sEV among BLV-infected cattle, whereas 118 proteins were uniquely expressed compared with those from uninfected cattle. Twenty-six proteins in milk sEV were differentially expressed proteins more than two-fold significant difference (p < 0.05) in BLV-infected cattle. GO and KEGG analyses indicated that the candidates for uniquely or differentially expressed proteins in milk sEV had been involved in diverse biological activities including metabolic processes, cellular processes, respond to stimulus, binding, catalytic activities, cancer pathways, focal adhesion, and so on. Taken together, the present findings provided a novel insight into the proteomes of milk sEV from BLV-infected cattle.
牛奶小型细胞外囊泡（sEV）中含有蛋白质，这些蛋白质提供了宿主生理学和免疫学的潜在信息。牛白血病病毒（BLV）是一种致癌病毒，可导致牛的进行性 B 细胞淋巴肉瘤。在本研究中，我们旨在探索 BLV 感染牛的牛奶 sEV 的蛋白质组学特征与未感染牛的牛奶 sEV 的蛋白质组学特征。从 3 头 BLV 感染牛和 3 头未感染牛中分离牛奶 sEV。采用全面的纳升液相色谱-串联质谱（nanoLC-MS/MS）方法进行蛋白质组学分析。此外，还使用基因本体论（GO）注释和京都基因与基因组百科全书（KEGG）通路来评估 BLV 感染牛的牛奶 sEV 中独特或差异表达蛋白的候选物。蛋白质组学分析显示，BLV 感染牛的牛奶 sEV 中有 1330 种共同蛋白质，而与未感染牛的牛奶 sEV 相比，有 118 种蛋白质是独特表达的。在 BLV 感染牛的牛奶 sEV 中，有 26 种蛋白质是差异表达蛋白，差异倍数大于两倍，具有统计学意义（p<0.05）。GO 和 KEGG 分析表明，牛奶 sEV 中独特或差异表达蛋白的候选物参与了多种生物学活动，包括代谢过程、细胞过程、对刺激的反应、结合、催化活性、癌症途径、焦点粘连等。总之，本研究结果为 BLV 感染牛的牛奶 sEV 的蛋白质组学提供了新的见解。
