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功能分析和基因组挖掘揭示了复杂环境中根瘤内细菌在生物防治和促进植物生长方面的巨大潜力。

Functional Analysis and Genome Mining Reveal High Potential of Biocontrol and Plant Growth Promotion in Nodule-Inhabiting Bacteria Within Complex.

作者信息

Ali Md Arshad, Lou Yang, Hafeez Rahila, Li Xuqing, Hossain Afsana, Xie Ting, Lin Li, Li Bin, Yin Yanni, Yan Jianli, An Qianli

机构信息

State Key Laboratory of Rice Biology, Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang Province Key Laboratory of Biology of Crop Pathogens and Insects, Institute of Biotechnology, College of Agricultural and Biotechnology, Zhejiang University, Hangzhou, China.

Hangzhou Academy of Agricultural Sciences, Hangzhou, China.

出版信息

Front Microbiol. 2021 Jan 18;11:618601. doi: 10.3389/fmicb.2020.618601. eCollection 2020.

DOI:10.3389/fmicb.2020.618601
PMID:33537018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7848036/
Abstract

Bacteria belonging to the genus were frequently isolated from legume nodules. The nodule-inhabiting as a resource of biocontrol and plant growth-promoting endophytes has rarely been explored. This study explored the nodule-inhabiting ' antifungal activities and biocontrol potentials against broad-spectrum important phytopathogenic fungi. We collected strains which were isolated from nodules of , , , , , or and belong to , , , , , , or closely related to , or . These nodule-inhabiting showed diverse antagonistic activities against five phytopathogenic fungi (, , , , and ). Six strains within the complex showed broad-spectrum and potent activities against all the five pathogens, and produced multiple hydrolytic enzymes, siderophores, and lipopeptide fusaricidins. Fusaricidins are likely the key antimicrobials responsible for the broad-spectrum antifungal activities. The nodule-inhabiting strains within the complex were able to epiphytically and endophytically colonize the non-host wheat plants, produce indole acetic acids (IAA), and dissolve calcium phosphate and calcium phytate. strains RP20, RP51, and RP62 could fix N. RP51 and sp. RP31, which showed potent plant colonization and plant growth-promotion competence, effectively control fungal infection . Genome mining revealed that all strains ( = 76) within the complex contain gene encoding indole-3-pyruvate decarboxylase for biosynthesis of IAA, 96% ( = 73) contain the cluster for biosynthesis of fusaricidins, and 43% ( = 33) contain the cluster for nitrogen fixation. Together, our study highlights that endophytic strains within the complex have a high probability to be effective biocontrol agents and biofertilizers and we propose an effective approach to screen strains within the complex.

摘要

属于该属的细菌经常从豆科植物根瘤中分离得到。作为生物防治和促进植物生长的内生菌资源，栖息于根瘤中的该属细菌很少被研究。本研究探索了栖息于根瘤中的该属细菌对广谱重要植物病原真菌的抗真菌活性和生物防治潜力。我们收集了从苜蓿、大豆、豌豆、三叶草、百脉根或紫云英的根瘤中分离得到的、属于该属、该属或与该属密切相关或属于该属或该属的菌株。这些栖息于根瘤中的该属细菌对五种植物病原真菌（立枯丝核菌、尖孢镰刀菌、腐皮镰刀菌、禾谷镰刀菌和灰葡萄孢）表现出不同的拮抗活性。该属复合体中的六个菌株对所有五种病原菌表现出广谱且强效的活性，并产生多种水解酶、铁载体和脂肽类杀镰孢菌素。杀镰孢菌素可能是负责广谱抗真菌活性的关键抗菌物质。该属复合体中的栖息于根瘤的菌株能够在非寄主小麦植物上进行附生和内生定殖，产生吲哚乙酸（IAA），并溶解磷酸钙和植酸钙。该属菌株RP20、RP51和RP62能够固氮。表现出强大的植物定殖和促进植物生长能力的RP51和该属菌株RP31有效控制了真菌感染。基因组挖掘表明，该属复合体中的所有菌株（n = 76）都含有编码用于IAA生物合成的吲哚-3-丙酮酸脱羧酶的基因，96%（n = 73）含有用于杀镰孢菌素生物合成的基因簇，43%（n = 33）含有用于固氮的基因簇。总之，我们研究强调该属复合体中的内生菌株很有可能成为有效的生物防治剂和生物肥料，并且我们提出了一种筛选该属复合体中菌株的有效方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d07/7848036/82812510ecc8/fmicb-11-618601-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d07/7848036/82812510ecc8/fmicb-11-618601-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d07/7848036/4d691b5cb375/fmicb-11-618601-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d07/7848036/c6452cfcd096/fmicb-11-618601-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3d07/7848036/82812510ecc8/fmicb-11-618601-g007.jpg

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