Agricen Sciences, Pilot Point Texas, USA.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1463-1469. doi: 10.1099/ijsem.0.003672.
Strain MS2379 was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas. Phylogenetic analyses based on both 16S rRNA gene and gene sequences showed that MS2379 was most closely related to (DSM 36) (DSM 13815) (DSM 8320) yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively. Rod-shaped cells of strain MS2379 are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores. The peptidoglycan contains -diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C (61.9 %) and anteiso-C (11.6 %), confirming that strain MS2379 has diagnostic features of other species. The G+C content of MS2379 is 45.9 mol%. Fermentation of glucose yields acid and gas end-products. The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine. The growth range of MS2379 was observed from 10-45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl. Unlike the most closely related species, strain MS2379 was negative in the Voges-Proskauer reaction. Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379. Thus, strain MS2379 represents a novel species of the genus for which the name sp. nov. is proposed with the type strain MS2379 (=DSM 107750=ATCC TSD-165).
MS2379 菌株是从德克萨斯州皮奥特莱特主要厌氧发酵系统处理牛粪的巴氏消毒溶液样本中分离出来的。基于 16S rRNA 基因和 基因序列的系统发育分析表明，MS2379 与 （DSM 36）（DSM 13815）（DSM 8320）最为密切相关，但通过 DNA-DNA 杂交进行的 DNA-DNA 相关性研究表明，与这三个物种的相关性分别仅为 22.6%、32.0%和 24.7%。MS2379 菌株的杆状细胞革兰氏染色可变，具有末端、椭圆形、变形的内生孢子。肽聚糖含有 -二氨基庚二酸（mDAP），主要脂肪酸为anteiso-C（61.9%）和anteiso-C（11.6%），这证实了 MS2379 菌株具有其他 物种的诊断特征。MS2379 的 G+C 含量为 45.9mol%。葡萄糖发酵产生酸和气体产物。发现的极性脂类有双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和糖脂，但也包括一些未鉴定的脂类、胺脂类、氨基糖脂类和磷脂酰甲乙醇胺。MS2379 的生长范围为 10-45°C，最适生长温度为 30°C。在 pH 值为 6-10 和高达 3%的 NaCl 下观察到生长。与最密切相关的 物种不同，MS2379 菌株在 Voges-Proskauer 反应中呈阴性。核酸、化学生物分类学和生物化学特征支持 MS2379 菌株的独特性。因此，MS2379 菌株代表了 属的一个新种，提议使用 MS2379 菌株（=DSM 107750=ATCC TSD-165）作为新种的模式菌株。
