Lubahn D B, Joseph D R, Sullivan P M, Willard H F, French F S, Wilson E M
Department of Pediatrics, University of North Carolina, Chapel Hill 27599.
Science. 1988 Apr 15;240(4850):327-30. doi: 10.1126/science.3353727.
The androgen receptor (AR) mediates the actions of male sex steroids. Human AR genomic DNA was cloned from a flow-sorted human X chromosome library by using a consensus nucleotide sequence from the DNA-binding domain of the family of nuclear receptors. The AR gene was localized on the human X chromosome between the centromere and q13. Cloned complementary DNA, selected with an AR-specific oligonucleotide probe, was expressed in monkey kidney (COS) cells and yielded a high-affinity androgen-binding protein with steroid-binding specificity corresponding to that of native AR. A predominant messenger RNA species of 9.6 kilobases was identified in human, rat, and mouse tissues known to contain AR and was undetectable in tissues lacking AR androgen-binding activity, including kidney and liver from androgen-insensitive mice. The deduced amino acid sequence of AR within the DNA-binding domain has highest sequence identity with the progesterone receptor.
雄激素受体（AR）介导雄性甾体激素的作用。通过使用核受体家族DNA结合域的共有核苷酸序列，从流式分选的人类X染色体文库中克隆了人类AR基因组DNA。AR基因定位于人类X染色体着丝粒和q13之间。用AR特异性寡核苷酸探针筛选出的克隆互补DNA在猴肾（COS）细胞中表达，产生了一种高亲和力雄激素结合蛋白，其类固醇结合特异性与天然AR的一致。在已知含有AR的人类、大鼠和小鼠组织中鉴定出一种主要的9.6千碱基信使RNA，而在缺乏AR雄激素结合活性的组织中无法检测到，包括雄激素不敏感小鼠的肾脏和肝脏。DNA结合域内AR的推导氨基酸序列与孕酮受体具有最高的序列同一性。
