Arm J P, Horton C E, Mencia-Huerta J M, House F, Eiser N M, Clark T J, Spur B W, Lee T H
Department of Medicine, United Medical School, Guy's Hospital, London.
Thorax. 1988 Feb;43(2):84-92. doi: 10.1136/thx.43.2.84.
Recruitment of inflammatory leucocytes to the airways may play a part in the pathogenesis of asthma. As dietary enrichment with fish oil lipids can suppress leucocyte function, the effect of these lipids on asthma control and neutrophil function was studied in 20 subjects with mild asthma. Twelve subjects received capsules containing 3.2 g of eicosapentaenoic acid and 2.2 g of docosahexaenoic acid daily and eight subjects received placebo capsules containing olive oil for 10 weeks in a double blind fashion. Baseline specific airways conductance, airways responsiveness to histamine and exercise, diurnal peak expiratory flow, symptom scores, and bronchodilator use were measured. Neutrophil fatty acid composition was evaluated by gas chromatography, calcium ionophore induced neutrophil leukotriene (LT)B4 and LTB5 generation were measured by reverse phase high performance liquid chromatography and radioimmunoassay, and neutrophil chemotactic responses to formyl-methionyl-leucyl-phenylalanine (FMLP) and LTB4 were assessed by a microchemotaxis technique. Although the fish oil supplemented diet produced a greater than 10 fold increase in the eicosapentaenoic acid content of neutrophil phospholipids, there was no significant change in airways responsiveness to histamine or any change in any of the clinical measurements. After dietary supplementation with fish oil there was a 50% inhibition of total LTB (LTB4 + LTB5) generation by ionophore stimulated neutrophils and neutrophil chemotaxis was substantially suppressed. Neutrophil function remained unchanged in the placebo group. It is concluded that in subjects with mild asthma a fish oil enriched diet attenuates neutrophil function without changing the severity of asthma.
炎症白细胞募集到气道可能在哮喘发病机制中起作用。由于用鱼油脂质丰富饮食可抑制白细胞功能，因此在20名轻度哮喘患者中研究了这些脂质对哮喘控制和中性粒细胞功能的影响。12名受试者每天接受含有3.2克二十碳五烯酸和2.2克二十二碳六烯酸的胶囊，8名受试者以双盲方式接受含有橄榄油的安慰剂胶囊，为期10周。测量了基线特异性气道传导率、气道对组胺和运动的反应性、日间呼气峰值流速、症状评分和支气管扩张剂使用情况。通过气相色谱法评估中性粒细胞脂肪酸组成，通过反相高效液相色谱法和放射免疫测定法测量钙离子载体诱导的中性粒细胞白三烯（LT）B4和LTB5生成，并通过微量趋化技术评估中性粒细胞对甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）和LTB4的趋化反应。尽管补充鱼油的饮食使中性粒细胞磷脂中二十碳五烯酸含量增加了10倍以上，但气道对组胺的反应性没有显著变化，任何临床测量指标也没有变化。补充鱼油饮食后，离子载体刺激的中性粒细胞产生的总LTB（LTB4 + LTB5）受到50%的抑制，中性粒细胞趋化性也被显著抑制。安慰剂组中性粒细胞功能保持不变。结论是，在轻度哮喘患者中，富含鱼油的饮食可减弱中性粒细胞功能，而不改变哮喘的严重程度。
