Miranda D T S Z, Zanatta A L, Miles E A, Calder P C, Nishiyama A
Departamento de Fisiologia, Centro Politécnico, Universidade Federal do Paraná, Centro Politécnico, Jardim das Américas, CEP 81531-990, Curitiba, Brazil.
School of Human Development & Health Academic Unit, Faculty of Medicine, University of Southampton, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, United Kingdom.
Heliyon. 2021 Nov 5;7(11):e08326. doi: 10.1016/j.heliyon.2021.e08326. eCollection 2021 Nov.
This study aimed to evaluate the levels of eicosanoids derived from arachidonic acid (ARA) in the lungs of asthmatic rats supplemented with fish oil. The present data gives insight into the action of fish oil in asthma, related to its inability to modify the contractile capacity of tracheal smooth muscle reported previously in a model of asthma in rats. Male Wistar rats were supplemented daily with 1 g of fish oil/kg of body weight for 21 days. They were exposed to ovalbumin (OVA) after previous sensitization with OVA to induce asthma. Pulmonary levels of five eicosanoids were measured using immunoassay kits: PGE, TXB, LTB, LXA, and 8-iso PGF. In asthmatic rats, supplementation with fish oil resulted in lower concentrations of lung eicosanoids produced by cyclooxygenase-2 and 15-lipoxygenase: PGE, TXB, and LXA, respectively. Fish oil supplementation also decreased the non-enzymatically produced eicosanoid 8-iso PGF. Fish oil supplementation did not affect LTB, a metabolite of 5-lipoxygenase. The limited efficacy of fish oil supplementation in asthmatic rats is associated with a lack of action in reducing the levels of LTB in the lungs. Thus, fish oil differentially modulates the concentrations of eicosanoids derived from ARA via specific pathways in an animal model of asthma.
本研究旨在评估补充鱼油的哮喘大鼠肺中源自花生四烯酸（ARA）的类二十烷酸水平。目前的数据揭示了鱼油在哮喘中的作用，这与之前在大鼠哮喘模型中报道的其无法改变气管平滑肌收缩能力有关。雄性Wistar大鼠每天补充1 g鱼油/千克体重，持续21天。在用卵清蛋白（OVA）预先致敏以诱导哮喘后，将它们暴露于OVA。使用免疫分析试剂盒测量五种类二十烷酸的肺水平：前列腺素E（PGE）、血栓素B（TXB）、白三烯B（LTB）、白三烯A（LXA）和8-异前列腺素F（8-iso PGF）。在哮喘大鼠中，补充鱼油导致环氧化酶-2和15-脂氧合酶产生的肺类二十烷酸浓度降低，分别为PGE、TXB和LXA。补充鱼油还降低了非酶促产生的类二十烷酸8-iso PGF。补充鱼油不影响5-脂氧合酶的代谢产物LTB。补充鱼油在哮喘大鼠中的疗效有限与降低肺中LTB水平缺乏作用有关。因此，在哮喘动物模型中，鱼油通过特定途径差异调节源自ARA的类二十烷酸浓度。
