Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
J Mol Biol. 2021 Mar 19;433(6):166861. doi: 10.1016/j.jmb.2021.166861. Epub 2021 Feb 1.
Almost all nucleoprotein interactions and DNA manipulation events involve mechanical deformations of DNA. Extraordinary progresses in single-molecule, structural, and computational methods have characterized the average mechanical properties of DNA, such as bendability and torsional rigidity, in high resolution. Further, the advent of sequencing technology has permitted measuring, in high-throughput, how such mechanical properties vary with sequence and epigenetic modifications along genomes. We review these recent technological advancements, and discuss how they have contributed to the emerging idea that variations in the mechanical properties of DNA play a fundamental role in regulating, genome-wide, diverse processes involved in chromatin organization.
几乎所有核蛋白相互作用和 DNA 操作事件都涉及 DNA 的机械变形。单分子、结构和计算方法的非凡进展已经以高分辨率描述了 DNA 的平均机械性质，例如柔韧性和扭转刚度。此外，测序技术的出现使得能够高通量测量这种机械性质如何随序列和基因组中表观遗传修饰而变化。我们回顾了这些最近的技术进步，并讨论了它们如何促成了一个新兴观点，即 DNA 机械性质的变化在调节染色质组织中涉及的广泛过程方面起着根本性作用。
