National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, PR China.
Bioresour Technol. 2021 Apr;326:124744. doi: 10.1016/j.biortech.2021.124744. Epub 2021 Jan 20.
Cell immobilization was used to enrich and retain functional bacteria within partial denitrification-Anammox (PD/A) process to achieve its fast start-up for the first time. To do so, residue sludge and Anammox sludge were immobilized in poly (vinyl alcohol)/sodium alginate (PVA/SA) gel for PD cultivation and Anammox bacteria inoculation, respectively. Stable PD with NO-N to NO-N transformation ratio (NTR) of 72.0% was achieved within 13 days at 25 °C and successfully combined with Anammox on 14th day. The hydrous porous PVA/SA gel matrix played the role of extracellular polymeric substance (EPS) and thus protected the microbes against low temperature. Satisfactory nitrogen removal rate (NRR) (301.6 ± 6.1 g N/(m·d)) was achieved even when temperature decreased to 13 °C. The contribution of nitrogen removal via Anammox was as high as 77.10%. Abundance of Thauera and Candidatus Kuenenia increased from 0.9% and 1.1% to 30.6% and 2.1%, respectively.
细胞固定化技术首次被用于富集和保留部分反硝化-厌氧氨氧化（PD/A）工艺中的功能细菌，以实现其快速启动。为此，将剩余污泥和厌氧氨氧化污泥分别固定在聚乙烯醇/海藻酸钠（PVA/SA）凝胶中，用于 PD 培养和接种厌氧氨氧化细菌。在 25°C 下，13 天内实现了稳定的 PD，NO-N 到 NO-N 的转化率（NTR）达到 72.0%，并在第 14 天成功与厌氧氨氧化结合。含水多孔 PVA/SA 凝胶基质起到了胞外聚合物（EPS）的作用，从而保护微生物免受低温影响。即使温度降至 13°C，仍能达到令人满意的氮去除率（NRR）（301.6±6.1 g N/（m·d））。通过厌氧氨氧化去除的氮的贡献高达 77.10%。Thauera 和 Candidatus Kuenenia 的丰度分别从 0.9%和 1.1%增加到 30.6%和 2.1%。
