Postdoctoral Research Base, Postdoctoral Innovation & Practice Base, Henan Institute of Science and Technology, Xinxiang 453003, China; Postdoctoral Research Station, Henan Agricultural University, Zhengzhou 450002, China; School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China; Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement, Xinxiang 453003, China.
School of Life Science and Technology, Henan Collaborative Innovation Center in Modern Biological Breeding, Henan Institute of Science and Technology, Xinxiang 453003, China.
Ecotoxicol Environ Saf. 2021 Apr 1;212:111998. doi: 10.1016/j.ecoenv.2021.111998. Epub 2021 Feb 2.
Metabolomics is an implement for testing the toxicity of antibiotics, and provides a comprehensive view of the overall response to stress; however, the connections between metabolites and biologic endpoints keep unclear in response to antibiotics. In this study, wheat seeds were exposed to tetracycline for 5 days. The results proved that tetracycline restrained growth, reduced chlorophyl and carotinoid contents and cell permeability, and increased reactive oxygen species (ROS) levels and malondialdehyde (MDA) content. Orthogonal partial least squares (OPLS) was used to analyze the connections between metabolites and biologic endpoints, which discovered that 11 metabolic pathways were significantly affected by tetracycline, and amino acid metabolism could largely apply to root growth and ROS accumulation, while carbohydrate metabolism could have a ruling effect on tetracycline-induced cell permeability. 13 metabolites all played active roles in mediating tetracycline's effects on root length, root fresh weight and cell permeability but had no significant effects on ROS levels. The majority of metabolites with passive effects on root length, root fresh weight and cell permeability had active effects on ROS levels. These results offer a view about stress reaction of wheat to tetracycline.
代谢组学是一种用于测试抗生素毒性的工具，它提供了对整体应激反应的全面观察；然而，在抗生素的作用下，代谢物与生物学终点之间的联系仍然不清楚。在这项研究中，小麦种子暴露于四环素中 5 天。结果表明，四环素抑制了生长，降低了叶绿素和类胡萝卜素的含量以及细胞通透性，并增加了活性氧（ROS）水平和丙二醛（MDA）的含量。正交偏最小二乘（OPLS）用于分析代谢物与生物学终点之间的关系，发现四环素显著影响了 11 条代谢途径，其中氨基酸代谢可在很大程度上应用于根的生长和 ROS 的积累，而碳水化合物代谢可对四环素诱导的细胞通透性产生主要影响。13 种代谢物都在调节四环素对根长、根鲜重和细胞通透性的影响方面发挥了积极作用，但对 ROS 水平没有显著影响。对根长、根鲜重和细胞通透性具有被动影响的大多数代谢物对 ROS 水平具有积极影响。这些结果提供了一个关于小麦对四环素应激反应的视角。
