Ramos M, Sánchez R M, Olano A, Sanz J, Martinez-Castro I
Instituto de Fermentaciones Industriales, C.S.I.C., Madrid, Spain.
Z Lebensm Unters Forsch. 1988 Jan;186(1):22-4. doi: 10.1007/BF01027174.
"Acid-stable, heat-stable polypeptides" (previously called proteose-peptone) of three milk species have been studied. Analysis by polyacrylamide gel electrophoresis revealed that all fractions showed similar patterns. Only bands with the lowest mobilities showed the presence of carbohydrates when stained with Schiff's reagent. These bands had a lower mobility in ewe's and goat's milk than in cow's milk. Gas chromatography/mass spectrometry analysis showed the presence of fucose, mannose, glucose, galactose, glucosamine and galactosamine in the three fractions studied. Acid-stable, heat-stable polypeptides of bovine milk had the lowest carbohydrate content (4 mg/100 mg protein), whereas the highest content was found in ewe's milk (7.30 mg/100 mg protein) mainly as a result of the high galactose, mannose and glucosamine content. The sialic acid content was lower in ewe's and goat's milk (2.68 and 2.98 mg/100 mg protein respectively) than in bovine milk (2.06 mg/100 mg protein).
对三种奶类的“酸稳定、热稳定多肽”（以前称为脉胨）进行了研究。聚丙烯酰胺凝胶电泳分析表明，所有组分都呈现出相似的图谱。用席夫试剂染色时，只有迁移率最低的条带显示出碳水化合物的存在。这些条带在羊奶和山羊奶中的迁移率低于牛奶。气相色谱/质谱分析表明，在所研究的三个组分中均存在岩藻糖、甘露糖、葡萄糖、半乳糖、氨基葡萄糖和半乳糖胺。牛奶的酸稳定、热稳定多肽的碳水化合物含量最低（4毫克/100毫克蛋白质），而羊奶中的含量最高（7.30毫克/100毫克蛋白质），这主要是由于半乳糖、甘露糖和氨基葡萄糖含量较高。羊奶和山羊奶中的唾液酸含量（分别为2.68和2.98毫克/100毫克蛋白质）低于牛奶（2.06毫克/100毫克蛋白质）。
