Nezamabadi Vida, Akhgar Mohammad Reza, Tahamipour Batool, Rajaei Peyman
Department of Chemistry, Faculty of Science, Kerman Branch, Islamic Azad University, Kerman, Iran.
Young Researchers and Elite Club, Sirjan Branch, Islamic Azad University, Sirjan, Iran.
Iran J Biotechnol. 2020 Apr 1;18(2):e2426. doi: 10.30498/IJB.2020.151379.2426. eCollection 2020 Apr.
Green approach to nanoparticles, including metal oxides Because of an inevitable disadvantage of physical or chemical synthesis routes is attractive nowadays. ZnO nanoparticles play a key role in the medicals and drugs area.
In this study, biosynthesis of ZnO nanoparticles with new approach to enhanced the Antimicrobial properties against gram-negative and gram-positive was performed by use of a new type of plant extract, , in an environmentally friendly, cost-effective, simple procedure way.
By adding Zn(NO) to methanol extract followed by stirring The resulted solution and final heat treatment in 200 °C the ZnO nanoparticles were synthesized. Disc diffusion method was applied to evaluation the Antimicrobial properties of the extract and nanoparticles towards resistance into (gram-negative) and (gram-positive).
X-ray diffraction pattern (XRD) result showed all of the peaks proportion to ZnO and no other peaks were detected, also demonstrated nanostructure nature with crystallite size about 9 nm. In the Fourier transform infrared spectroscopy (FTIR), there is a band in the 550 cm which is corresponded to ZnO. Also 76 nm average particle size obtained by DLS experiments. Energy-dispersive X-ray spectroscopy (EDS) analysis showed strong peaks for Zn and O, support supposition of ZnO nanoparticles. Field emission scanning electron microscopy (FESEM) images indicated spherical rounded particles with the size of average 30 nm. Antibacterial tests showed effective diameter about 11 and 10 mm for plant extract and also 7 and 5 mm for ZnO nanoparticles against (gram-negative) and (gram-positive) in agar disc diffusion method, respectively.
Biosynthesized ZnO nanoparticles could be a good candidate for antibacterial activity, both against (gram-negative) and (gram-positive) especially for versus .
纳米颗粒的绿色制备方法，包括金属氧化物，由于物理或化学合成路线存在不可避免的缺点，如今备受关注。氧化锌纳米颗粒在医疗和药物领域发挥着关键作用。
在本研究中，采用一种新型植物提取物，以环保、经济高效且简单的程序方式，通过新方法生物合成氧化锌纳米颗粒，以增强其对革兰氏阴性菌和革兰氏阳性菌的抗菌性能。
向甲醇提取物中加入硝酸锌，搅拌后，将所得溶液在200°C下进行最终热处理，合成氧化锌纳米颗粒。采用纸片扩散法评估提取物和纳米颗粒对大肠杆菌（革兰氏阴性菌）和金黄色葡萄球菌（革兰氏阳性菌）的抗菌性能。
X射线衍射图谱（XRD）结果显示所有峰均与氧化锌对应，未检测到其他峰，同时表明其具有约9nm微晶尺寸的纳米结构性质。在傅里叶变换红外光谱（FTIR）中，550cm-1处有一个与氧化锌对应的谱带。通过动态光散射（DLS）实验获得的平均粒径为76nm。能量色散X射线光谱（EDS）分析显示锌和氧的强峰，支持氧化锌纳米颗粒的假设。场发射扫描电子显微镜（FESEM）图像显示平均尺寸约为30nm的球形颗粒。抗菌测试表明，在琼脂纸片扩散法中，植物提取物对大肠杆菌（革兰氏阴性菌）和金黄色葡萄球菌（革兰氏阳性菌）的有效抑菌直径分别约为11mm和10mm，氧化锌纳米颗粒对它们的有效抑菌直径分别约为7mm和5mm。
生物合成的氧化锌纳米颗粒可能是一种对抗大肠杆菌（革兰氏阴性菌）和金黄色葡萄球菌（革兰氏阳性菌）具有抗菌活性的良好候选物，尤其是对大肠杆菌。
