评估氧化锌纳米颗粒对[具体细菌名称]中流出泵基因（）表达的影响。 需注意，原文中括号里“efflux pump genes ()”括号内内容缺失，不太完整准确。
Evaluation the effect of ZnO nanoparticle derived on the expression of efflux pump genes () in .
作者信息
Saleh Fatemeh, Kheirandish Farnaz, Hosseini Farzaneh, Yazdian Fatemeh
机构信息
Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran.
Department of Medical Parasitology and Mycology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
出版信息
J Environ Health Sci Eng. 2021 May 26;19(1):1133-1141. doi: 10.1007/s40201-021-00679-w. eCollection 2021 Jun.
PURPOSE
Green approach to the nanoparticles, including metal oxides due to inevitable disadvantages of physical or chemical synthesis routes is attractive nowadays. Zink oxide (ZnO) nanoparticles play a key role in the medical and pharmaceutical fields. This research aimed to study the biologically synthesized ZnO nanoparticle using , and evaluated its antibacterial properties.
METHODS
culture in a broth nutrient environment was used, followed by adding the Zinc acetate dehydrate. Biosynthesis of the nanoparticles was confirmed by the XRD, FTIR, and SEM imaging. The antibacterial effects of NPs on the expression of efflux pump genes and the regulator were studied; clinical species of the Acinetobacter were collected from clinical samples of Khorramabad, using the phenotypic (MIC) and the genotypic methods through real-time PCR.
RESULTS
X-ray diffraction pattern (XRD) result showed, that all of the peaks were related to the ZnO, and no other peaks were detected; it also demonstrated nanostructure nature with crystallite size of 25-50 nm. The results indicated, that the antibacterial properties of the nanoparticle increased the expression and decreased the expression in 40% of the In addition, there was an increase in the expression in 60% of the species, indicating an increased probability for mutation.
CONCLUSION
Given the desirable inhibitory effects of biosynthesized ZnO NPs on the expression of AdeB and AdeRS, which play an important role in the pharmaceutical resistance of species, it seems that ZnO NPs can be used as a medication candidate in pharmaceutical industry in the future.
目的
由于物理或化学合成路线不可避免的缺点，绿色合成纳米颗粒（包括金属氧化物）的方法如今备受关注。氧化锌（ZnO）纳米颗粒在医学和制药领域发挥着关键作用。本研究旨在利用[具体内容缺失]生物合成ZnO纳米颗粒，并评估其抗菌性能。
方法
采用在肉汤营养环境中培养，随后添加醋酸锌二水合物。通过X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）成像确认纳米颗粒的生物合成。研究了纳米颗粒对流出泵基因表达和[具体基因缺失]调节因子的抗菌作用；从霍拉马巴德的临床样本中收集不动杆菌的临床菌株，通过实时聚合酶链反应（PCR）使用表型（最小抑菌浓度，MIC）和基因型方法。
结果
X射线衍射图谱（XRD）结果显示，所有峰均与ZnO相关，未检测到其他峰；还证明了其纳米结构性质，微晶尺寸为25 - 50纳米。结果表明，纳米颗粒的抗菌性能使40%的[具体菌种缺失]中[具体基因缺失]的表达增加，[另一具体基因缺失]的表达降低。此外，60%的菌种中[具体基因缺失]的表达增加，表明突变概率增加。
结论
鉴于生物合成的ZnO纳米颗粒对在[具体菌种缺失]的药物抗性中起重要作用的AdeB和AdeRS表达具有理想的抑制作用，未来ZnO纳米颗粒似乎可作为制药行业的候选药物。
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