Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
Department of Chemical Engineering and Technology, Indian Institute of Technology (IIT-BHU), Varanasi, Uttar Pradesh, 221005, India.
Environ Sci Pollut Res Int. 2021 Nov;28(42):58857-58871. doi: 10.1007/s11356-021-12609-2. Epub 2021 Feb 5.
Green macroalgae comprise significant amount of structural carbohydrates for their conversion to liquid biofuels. However, it generally relies on species characteristics and the variability in seasonal profile to determine its route for bioprocessing. Hence, this study was conducted to analyze the indigenous marine macroalgal strain (Ulva prolifera) with respect to periodic trend and reducing sugar extraction. Consequently, in our investigation, the monthly variation in sugar profile and bioethanol yield was assessed between the monsoon and post-monsoon seasons, of which relatively high reducing sugar and fermentative bioethanol yield of about 0.152 ± 0.009 g/gdw and 6.275 ± 0.161 g/L was obtained for the October-month isolate (MITM10). Thereafter, the biochemical profile of this collected biomass (MITM10) revealed carbohydrate 34.98 ± 3.30%, protein 12.45 ± 0.49%, and lipid 1.93 ± 0.07%, respectively, on dry weight basis. Of these, the total carbohydrate fraction yielded the maximum reducing sugar of 0.156 ± 0.005 g/gdw under optimal conditions (11.07% (w/v) dosage, 0.9 M HSO, 121°C for 50 min) for thermal-acid hydrolysis. Furthermore, the elimination of polysaccharides was confirmed using the characterization techniques scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy. Therefore, the present thermochemical treatment method provides a species-specific novel strategy to breakdown the macroalgal cell wall polysaccharides that enhances sugar extraction for its utilization as an efficient bioenergy resource.
绿藻含有大量的结构性碳水化合物，可将其转化为液体生物燃料。然而，它通常依赖于物种特性和季节性变化来确定其生物加工途径。因此，本研究旨在分析本土海洋大型藻类菌株（Ulvaprolifera）的周期性趋势和还原糖提取。因此，在我们的研究中，评估了季风前和季风后季节之间的糖谱和生物乙醇产量的月变化，其中 10 月份分离株（MITM10）获得了相对较高的还原糖和发酵生物乙醇产量，分别为 0.152±0.009g/gdw 和 6.275±0.161g/L。随后，对该收集生物质（MITM10）的生化特性进行了分析，结果表明干重基础上的碳水化合物含量为 34.98±3.30%，蛋白质含量为 12.45±0.49%，脂质含量为 1.93±0.07%。其中，在最佳条件下（11.07%（w/v）剂量、0.9M HSO4、121°C 加热 50min），总碳水化合物部分的还原糖产量最高，为 0.156±0.005g/gdw。此外，还使用扫描电子显微镜（SEM）和傅里叶变换红外（FT-IR）光谱学等特征技术证实了多糖的消除。因此，本热化学处理方法为分解大型藻类细胞壁多糖提供了一种特定于物种的新策略，可提高糖的提取效率，将其用作有效的生物能源资源。
