Department of Cardiology, University of Medicine and Pharmacy of Craiova, Romania;
Rom J Morphol Embryol. 2020 Apr-Jun;61(2):521-528. doi: 10.47162/RJME.61.2.22.
The aim of our study was to assess histologically and by cardiac ultrasound the effects of alpha-lipoic acid (ALA) and vitamin B complex, as pathogenic therapies, in diabetic cardiomyopathy (DCM) in mice.
We performed an experimental animal study, in which we analyzed from a structural and functional point of view the changes produced in DCM. To produce DCM, we induced diabetes mellitus (DM) in C57BL∕6 mice by intraperitoneal injection of a single 150 mg∕kg body weight dose of streptozotocin (STZ). We formed a sham group (animals without DM), a control group (animals with DM but without treatment, DM_Control) and a group of animals with DM that were treated with ALA and vitamin B complex (DM_Treated).
At six weeks after STZ administration, there was no decrease in left ventricular ejection fraction (LVEF) in the sham group, while in the control group there was a significant decrease in LVEF, about 43.75±3.37%, compared to the group that received treatment with ALA and vitamin B complex, in which LVEF decreased to 49.6±5.02% (p=0.0432). Also, the degree of interstitial myocardial fibrosis was higher in animals with DM compared to animals without DM, but the applied therapeutic protocol considerably improved the accumulation of interstitial collagen. The same observation was maintained regarding the evaluation of polysaccharide deposits.
We can say that the administration of ALA and vitamin B complex in mice with STZ-induced DM, improves the degree of myocardial fibrosis, the accumulation of polysaccharides, and prevents severe deterioration of systolic and diastolic function of the heart.
本研究旨在通过心脏超声和组织学评估，研究α-硫辛酸（ALA）和维生素 B 复合物作为治疗药物对糖尿病心肌病（DCM）的作用。
我们进行了一项动物实验研究，从结构和功能角度分析 DCM 产生的变化。为了诱导 DCM，我们通过腹腔注射 150mg/kg 体重的链脲佐菌素（STZ）单次诱导 C57BL/6 小鼠发生糖尿病。我们设立了假手术组（无糖尿病的动物）、对照组（有糖尿病但未治疗的动物，DM_Control）和用 ALA 和维生素 B 复合物治疗的糖尿病组（DM_Treated）。
在 STZ 给药 6 周后，假手术组的左心室射血分数（LVEF）没有下降，而对照组的 LVEF 显著下降，约为 43.75±3.37%，与接受 ALA 和维生素 B 复合物治疗的组相比，LVEF 下降至 49.6±5.02%（p=0.0432）。此外，与无糖尿病的动物相比，糖尿病动物的心肌间质纤维化程度更高，但应用的治疗方案显著改善了间质胶原的积累。在多糖沉积的评估中也观察到了同样的结果。
我们可以说，在 STZ 诱导的糖尿病小鼠中给予 ALA 和维生素 B 复合物，可以改善心肌纤维化程度、多糖的积累，并防止心脏收缩和舒张功能的严重恶化。
