Oyewusi Adeoye Joshua, Oridupa Olayinka Ayotunde, Saba Adebowale Bernard, Oyewusi Ibironke Kofoworola, Olukunle Jonny Olufemi
Department of Veterinary Pharmacology and Toxicology, Federal University of Agriculture, Abeokuta, Nigeria.
Department of Veterinary Pharmacology and Toxicology, University of Ibadan, Ibadan, Nigeria.
J Basic Clin Physiol Pharmacol. 2021 Feb 8;32(6):1087-1092. doi: 10.1515/jbcpp-2020-0080.
Several cultivars of L. have been studied for anti-inflammatory and analgesic activities but there is inadequate information on such biological activities of the concentrated extracts of the Nigerian grown red cultivar bulb.
The anti-inflammatory models used in this study were Carrageenan-induced paw oedema and formalin-induced paw lick in rats, while acetic acid-induced abdominal writhing, hot plate reaction, hot water tail flick tests in mice were the analgesic models.
At 30 min post-induction (pi), the inhibition of paw oedema (62.50%) by 200 mg/kg of methanol extract of red cultivar bulb (MERCACB) was significantly (p<0.001) higher than that of indomethacin (15.63%) at 10 mg/kg. The paw oedema inhibition at 60 min pi by MERCACB (76.92%) was significantly higher than that of indomethacin (41.03%). At the early phase of formalin paw-lick test, the pain reaction time (PRT) of rat treated with MERCACB (400 mg/kg) was significantly lower than that of indomethacin and the control groups. The hotplate test revealed that PRT of mice treated with 800 mg/kg of MERCACB were significantly (p<0.01) longer in comparism to indomethacin and control groups. The PRT of mice subjected to thermal pain due to hot water and treated with 800 mg/kg of MERCACB was significantly (p<0.05) longer than that of the control group.
These findings indicate that MERCACB possesses potent anti-inflammatory and analgesic properties which confirm the traditional use of the plant for the treatment of inflammatory diseases and may be useful as a future therapeutic agent.
已对几种百合品种的抗炎和镇痛活性进行了研究，但关于尼日利亚种植的红色品种鳞茎浓缩提取物的此类生物活性的信息不足。
本研究中使用的抗炎模型为角叉菜胶诱导的大鼠足肿胀和福尔马林诱导的大鼠舔足，而小鼠的醋酸诱导扭体、热板反应、热水甩尾试验为镇痛模型。
诱导后30分钟时，200mg/kg红色品种鳞茎甲醇提取物（MERCACB）对足肿胀的抑制率（62.50%）显著高于10mg/kg吲哚美辛的抑制率（15.63%）（p<0.001）。诱导后60分钟时，MERCACB对足肿胀的抑制率（76.92%）显著高于吲哚美辛的抑制率（41.03%）。在福尔马林舔足试验的早期阶段，用MERCACB（400mg/kg）处理的大鼠的疼痛反应时间（PRT）显著低于吲哚美辛组和对照组。热板试验显示，与吲哚美辛组和对照组相比，用800mg/kg MERCACB处理的小鼠的PRT显著延长（p<0.01）。用800mg/kg MERCACB处理的因热水导致热痛的小鼠的PRT显著长于对照组（p<0.05）。
这些发现表明MERCACB具有强大的抗炎和镇痛特性，证实了该植物在治疗炎症性疾病方面的传统用途，可能作为未来的治疗药物。
