AGH University of Science and Technology, Faculty of Materials Science and Ceramics, A. Mickiewicz 30 Ave., 30-059 Krakow, Poland; Department of Chemical Engineering, Northeastern University, 360 Huntington Ave., Boston, MA, United States.
Department of Chemical Engineering, Northeastern University, 360 Huntington Ave., Boston, MA, United States.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111703. doi: 10.1016/j.msec.2020.111703. Epub 2020 Nov 11.
Physicochemical, electrochemical and biological performance of 4 types of all-carbon nanotube layers was studied. Higher oxidation state of carbon was responsible for micro-scaled uniformity of the layers and excellent electrical conductivity, while nitrogen containing functional groups yielded materials with anisotropy similar to natural tissues and reduced work function. All materials were cytocompatible with mammalian fibroblasts (viability >80%, cytotoxicity <3% at day 7) and human dermal fibroblast (viability of cells >70% at day 1), while reducing bacterial and cancer cells proliferation without adding any drug. After 8 h culture, a ~50% depletion in the number of Gram-positive bacteria was observed on materials with lower work function, while Gram-negative bacteria were more sensitive towards carbon coordination number and presence of nitrogen atoms (cell depletion of up to 48% on amidized carbon nanotubes). After 1-day culture, >80% reduction in the melanoma cells number, connected with enhanced production of reactive oxygen species (ROS) was observed. All-carbon nanotube layers decreased bacteria and cancer cell functions without negatively influencing mammalian cells nor using drugs and we believe that this can be explained by various sensitivity of the tested cells towards exogenous ROS overproduction. As the concerns over implant-related infections as well as rates of antibiotic-resistant bacteria and chemotherapeutic-resistant cancer cells are growing, such materials should pave the way for a wide range of biomedical applications.
研究了 4 种全碳纳米管层的物理化学、电化学和生物学性能。较高的碳氧化态负责使层具有微尺度均匀性和优异的导电性，而含氮官能团则产生具有类似天然组织各向异性和降低功函数的材料。所有材料均与哺乳动物成纤维细胞（第 7 天活力>80%，细胞毒性<3%）和人真皮成纤维细胞（第 1 天细胞活力>70%）相容，同时在不添加任何药物的情况下抑制细菌和癌细胞的增殖。培养 8 小时后，在功函数较低的材料上，革兰氏阳性菌的数量减少了约 50%，而革兰氏阴性菌对碳配位数和氮原子的存在更为敏感（在酰胺化碳纳米管上，细胞减少了 48%）。培养 1 天后，观察到黑素瘤细胞数量减少了 80%以上，这与活性氧（ROS）的产生增加有关。全碳纳米管层在不影响哺乳动物细胞、不使用药物的情况下降低了细菌和癌细胞的功能，我们认为这可以用测试细胞对外源 ROS 过度产生的不同敏感性来解释。由于对植入物相关感染以及抗生素耐药菌和化疗耐药癌细胞的担忧日益增加，此类材料应为广泛的生物医学应用铺平道路。
