Ru Xiangli, Liu Peng, Liu Tingting, Ma Xiaoming, Yang Lin
Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China; School of Environment, Henan Normal University, Xinxiang, Henan 453007, PR China.
Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111708. doi: 10.1016/j.msec.2020.111708. Epub 2020 Nov 6.
Modifying living cells using in-situ synthesized nanomaterials to endow them with new functions is highly desirable. Herein we report intra- and extra-cellular dual-modified red blood cells (RBCs) with intracellular CaCO nanoparticles (NPs) and extracellular polypyrrole-folic acid (PPy-FA) coating, which are exploited as a bifunctional drug carrier. The functionalized living cells (CaCO@RBC@PPy-FA) are fabricated through first the intracellular in situ reaction of exogenous Ca and CO ions to generate CaCO NPs, then polymerization of pyrrole and finally modification of folic acid (FA) on the membrane of individual cells, forming a CaCO@RBC@PPy-FA structure. As a result, such dual-modified RBCs not only preserve the original performances of the cells but also possess the desirable properties as a drug carrier, such as high loading capacity due to the action of CaCO NPs, targeting and light-controlled drug release due to the action of PPy-FA. Under NIR laser stimulation, these bifunctional RBCs (DOX-CaCO@RBC@PPy-FA) present an instant release profile of doxorubicin (DOX) and have high targeting-ability toward cancer cells, achieving a marked synergistic combined photothermal-chemotherapy effect.
利用原位合成的纳米材料修饰活细胞以赋予其新功能是非常理想的。在此，我们报道了细胞内和细胞外双重修饰的红细胞（RBC），其具有细胞内碳酸钙纳米颗粒（NPs）和细胞外聚吡咯-叶酸（PPy-FA）涂层，被用作双功能药物载体。功能化活细胞（CaCO@RBC@PPy-FA）的制备过程如下：首先通过外源钙和碳酸根离子的细胞内原位反应生成碳酸钙纳米颗粒，然后进行吡咯聚合，最后在单个细胞的膜上修饰叶酸（FA），形成CaCO@RBC@PPy-FA结构。结果，这种双重修饰的红细胞不仅保留了细胞的原始性能，还具有作为药物载体的理想特性，例如由于碳酸钙纳米颗粒的作用而具有高负载能力，由于聚吡咯-叶酸的作用而具有靶向性和光控药物释放能力。在近红外激光刺激下，这些双功能红细胞（DOX-CaCO@RBC@PPy-FA）呈现出阿霉素（DOX）的即时释放曲线，并且对癌细胞具有高靶向能力，实现了显著的光热-化疗协同联合效应。
