Jilin Provincial Precision Medicine Key Laboratory for Cardiovascular Genetic Diagnosis (Jilin Provincial Engineering Laboratory for Endothelial Function and Genetic Diagnosis of Cardiovascular Disease, Jilin Provincial Molecular Biology Research Center for Precision Medicine of Major Cardiovascular Disease, Jilin Provincial Cardiovascular Research Institute), Department of Cardiology, The Third Hospital of Jilin University, Changchun, China.
Medicine (Baltimore). 2021 Jan 22;100(3):e23468. doi: 10.1097/MD.0000000000023468.
The G0/G1 switch 2 (G0S2) gene is closely related to lipolysis, cell proliferation, apoptosis, oxidative phosphorylation, and the development of a variety of tumors. The aim of the present study was to expand the sample size to confirm the relationship between the expression of the G0S2 gene in peripheral blood and acute myocardial infarction (AMI) based on previous gene chip results.
Three hundred patients were initially selected, of which 133 were excluded in accordance with the exclusion criteria. Peripheral blood leukocytes were collected from 92 patients with AMI and 75 patients with stable coronary atherosclerotic disease (CAD). mRNA expression levels of G0S2 in peripheral blood leukocytes was measured by RT-PCR, and protein expression levels by Western blot analysis. The results of these assays in the 2 groups were compared.
mRNA expression levels of GOS2 in the peripheral blood leukocytes of patients with AMI were 0.41-fold lower than those of patients with stable CAD (P < .05), and GOS2 protein expression levels were 0.45-fold lower. Multivariate logistic regression analysis indicated that low expression levels of the G0S2 gene increased the risk of AMI by 2.08-fold in stable CAD patients.
G0S2 gene expression in the peripheral blood leukocytes of AMI patients was lower than that of stable CAD patients. Low G0S2 gene expression in peripheral blood leukocytes is an independent risk factor for AMI in stable CAD patients.
G0/G1 开关 2（G0S2）基因与脂肪分解、细胞增殖、细胞凋亡、氧化磷酸化以及多种肿瘤的发生发展密切相关。本研究旨在通过前期基因芯片结果，扩大样本量进一步证实外周血 G0S2 基因表达与急性心肌梗死（AMI）的关系。
最初选择了 300 例患者，根据排除标准排除了 133 例。采集 92 例 AMI 患者和 75 例稳定型冠状动脉粥样硬化性疾病（CAD）患者的外周血白细胞，采用 RT-PCR 检测外周血白细胞中 G0S2 的 mRNA 表达水平，采用 Western blot 分析检测蛋白表达水平。比较两组的检测结果。
AMI 患者外周血白细胞中 GOS2 的 mRNA 表达水平较稳定 CAD 患者低 0.41 倍（P＜.05），GOS2 蛋白表达水平低 0.45 倍。多因素 logistic 回归分析表明，稳定 CAD 患者中 G0S2 基因低表达使 AMI 的发病风险增加 2.08 倍。
AMI 患者外周血白细胞中 G0S2 基因表达低于稳定 CAD 患者。外周血白细胞中 G0S2 基因低表达是稳定 CAD 患者发生 AMI 的独立危险因素。
