Department of Agriculture, Forestry and Bioresources, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Korea Food Research Institute, Wanju-gun, Jeollabuk-do 55365, Korea.
Int J Mol Sci. 2021 Feb 3;22(4):1518. doi: 10.3390/ijms22041518.
Gray mold () is a fungal plant pathogen causing postharvest decay in strawberry fruit. Here, we conducted a comparative transcriptome analysis to identify differences in gene expression between the immature-green (IG) and mature-red (MR) stages of the "Sunnyberry" (gray mold-resistant) and "Kingsberry" (gray mold susceptible) strawberry cultivars. Most of the genes involved in lignin and alkane-type wax biosynthesis were relatively upregulated in "Sunnyberry". However, pathogenesis-related proteins encoding R- and antioxidant-related genes were comparatively upregulated in "Kingsberry". Analysis of gene expression and physiological traits in the presence and absence of inoculation revealed that the defense response patterns significantly differed between IG and MR rather than the cultivars. "Kingsberry" showed higher antioxidant induction at IG and upregulated hemicellulose-strengthening and R genes at MR. Hence, "Sunnyberry" and "Kingsberry" differed mainly in terms of the expression levels of the genes forming cuticle, wax, and lignin and controlling the defense responses. These discrepancies might explain the relative difference between these strawberry cultivars in terms of their postharvest responses to .
灰霉病（）是一种真菌植物病原体，可导致草莓果实采后腐烂。在这里，我们进行了比较转录组分析，以鉴定“阳光莓”（抗灰霉病）和“金莓”（易感灰霉病）草莓品种的未成熟绿色（IG）和成熟红色（MR）阶段之间基因表达的差异。木质素和烷烃型蜡生物合成中涉及的大多数基因在“阳光莓”中相对上调。然而，与 R 相关的蛋白编码和与抗氧化相关的基因在“金莓”中上调。在存在和不存在接种的情况下分析基因表达和生理特性表明，防御反应模式在 IG 和 MR 之间存在显著差异，而不是品种之间的差异。“金莓”在 IG 时表现出更高的抗氧化诱导作用，在 MR 时上调半纤维素强化和 R 基因。因此，“阳光莓”和“金莓”主要在形成表皮、蜡质和木质素的基因表达水平以及控制防御反应方面存在差异。这些差异可能解释了这两个草莓品种在其对灰霉病的采后反应方面的相对差异。
