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减毒活疫苗株（SG9R）的优化解毒以改进针对禽伤寒的疫苗策略。

Optimized Detoxification of a Live Attenuated Vaccine Strain (SG9R) to Improve Vaccine Strategy against Fowl Typhoid.

作者信息

Kim Nam-Hyung, Ko Dae-Sung, Ha Eun-Jin, Ahn Sunmin, Choi Kang-Seuk, Kwon Hyuk-Joon

机构信息

Laboratory of Poultry Medicine, College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.

Research Institute for Veterinary Science, College of Veterinary Medicine, BK21 for Veterinary Science, Seoul 08826, Korea.

出版信息

Vaccines (Basel). 2021 Feb 3;9(2):122. doi: 10.3390/vaccines9020122.

DOI:10.3390/vaccines9020122
PMID:33546449
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7913755/
Abstract

The live attenuated vaccine strain, SG9R, has been used against fowl typhoid worldwide, but it can revert to the pathogenic smooth strain owing to single nucleotide changes such as nonsense mutations in the gene. As SG9R possesses an intact plasmid with virulence genes, it exhibits dormant pathogenicity and can cause fowl typhoid in young chicks and stressed or immunocompromised brown egg-laying hens. To tackle these issues, we knocked out the gene of SG9R (named Safe-9R) to eliminate the reversion risk and generated detoxified strains of Safe-9R by knocking out , , , and genes to attenuate the virulence. Among the knockout strains, live Δ- (Dtx-9RL) and Δ-9R (Dtx-9RM) strains induced remarkably less expression of inflammatory cytokines in chicken macrophage cells, and oil emulsion (OE) Dtx-9RL did not cause body weight loss in chicks. Live Dtx-9RM exhibited efficacy against field strain challenge in one week without any bacterial re-isolation, while the un-detoxified strains showed the development of severe liver lesions and re-isolation of challenged strains. Thus, SG9R was optimally detoxified by knockout of and , and Dtx-9RL and Dtx-9RM might be applicable as OE and live vaccines, respectively, to prevent fowl typhoid irrespective of the age of chickens.

摘要

减毒活疫苗株SG9R已在全球范围内用于防治禽伤寒，但由于该基因中的单核苷酸变化（如无义突变），它可能会回复为致病的光滑型菌株。由于SG9R拥有一个带有毒力基因的完整质粒，它表现出潜伏致病性，可在幼雏以及应激或免疫功能低下的褐壳蛋鸡中引发禽伤寒。为解决这些问题，我们敲除了SG9R的基因（命名为Safe-9R）以消除回复风险，并通过敲除、、和基因来减弱毒力，从而构建了Safe-9R的解毒菌株。在这些敲除菌株中，活的Δ-（Dtx-9RL）和Δ-9R（Dtx-9RM）菌株在鸡巨噬细胞中诱导的炎性细胞因子表达显著降低，并且油乳剂（OE）Dtx-9RL不会导致雏鸡体重减轻。活的Dtx-9RM在一周内对野毒株攻击显示出有效性，且未出现任何细菌再分离现象，而未解毒的菌株则出现严重的肝脏病变并再次分离出攻击的菌株。因此，通过敲除和对SG9R进行了最佳解毒，Dtx-9RL和Dtx-9RM可能分别作为油乳剂疫苗和活疫苗适用于预防任何年龄鸡的禽伤寒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/39b5ab0dbfd5/vaccines-09-00122-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/d8cbface5392/vaccines-09-00122-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/aa62af41b0f5/vaccines-09-00122-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/94009f629da8/vaccines-09-00122-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/e156322451f6/vaccines-09-00122-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/fd66a26ff2c1/vaccines-09-00122-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/bb9a0093e2c5/vaccines-09-00122-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/39b5ab0dbfd5/vaccines-09-00122-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/d8cbface5392/vaccines-09-00122-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/aa62af41b0f5/vaccines-09-00122-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/94009f629da8/vaccines-09-00122-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/e156322451f6/vaccines-09-00122-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/fd66a26ff2c1/vaccines-09-00122-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/bb9a0093e2c5/vaccines-09-00122-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8b6/7913755/39b5ab0dbfd5/vaccines-09-00122-g007.jpg

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本文引用的文献

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2
Molecular evolution of Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum in the field.鸡白痢沙门氏菌野生物种在田间的分子进化。
Vet Microbiol. 2019 Aug;235:63-70. doi: 10.1016/j.vetmic.2019.05.019. Epub 2019 May 24.
3
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Animals (Basel). 2023 Apr 8;13(8):1284. doi: 10.3390/ani13081284.
4
Fowl typhoid live lyophilized vaccine applied at 3-month intervals protected layer chickens from infection and prevented cloacal shedding.每隔3个月接种一次的鸡伤寒活冻干疫苗可保护蛋鸡免受感染，并防止泄殖腔排菌。
J Adv Vet Anim Res. 2022 Jun 28;9(2):301-309. doi: 10.5455/javar.2022.i597. eCollection 2022 Jun.
5
Development of Next-Generation Vaccines.下一代疫苗的研发
Vaccines (Basel). 2022 Feb 10;10(2):274. doi: 10.3390/vaccines10020274.
鸡伤寒沙门氏菌的分子和系统发育分析追溯了家禽养殖场近期禽伤寒疫情的起源和多样性。
Vet Microbiol. 2017 Dec;212:80-86. doi: 10.1016/j.vetmic.2017.11.001. Epub 2017 Nov 17.
4
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