针对两种灭活全病毒疫苗（猫白血病病毒 Fel-O-Vax Lv-K 和 Fel-O-Vax 5）对猫进行接种后，针对猫免疫缺陷病毒的抗 SU 抗体反应。

Anti-SU Antibody Responses in Client-Owned Cats Following Vaccination against Feline Leukaemia Virus with Two Inactivated Whole-Virus Vaccines (Fel-O-Vax Lv-K and Fel-O-Vax 5).

机构信息

Sydney School of Veterinary Science, The University of Sydney, Camperdown, NSW 2006, Australia.

Centre for Veterinary Education, The University of Sydney, Camperdown, NSW 2006, Australia.

出版信息

Viruses. 2021 Feb 3;13(2):240. doi: 10.3390/v13020240.

DOI:10.3390/v13020240

PMID:33546485

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7913631/

Abstract

A field study undertaken in Australia compared the antibody responses induced in client-owned cats that had been vaccinated using two inactivated whole feline leukaemia virus (FeLV) vaccines, the monovalent vaccine Fel-O-Vax Lv-K and the polyvalent vaccine Fel-O-Vax 5. Serum samples from 428 FeLV-uninfected cats (118 FeLV-vaccinated and 310 FeLV-unvaccinated) were tested for anti-FeLV neutralising antibodies (NAb) using a live virus neutralisation assay to identify 378 FeLV-unexposed (NAb-negative) and 50 FeLV-exposed (NAb-positive; abortive infections) cats, following by anti-surface unit (SU) FeLV-A and FeLV-B antibody ELISA testing. An additional 42 FeLV-infected cats (28 presumptively regressively infected, 14 presumptively progressively infected) were also tested for anti-SU antibodies. NAb-positive cats displayed significantly higher anti-SU antibody ELISA responses compared to NAb-negative cats ( < 0.001). FeLV-unexposed cats (NAb-negative) that had been vaccinated less than 18 months after a previous FeLV vaccination using the monovalent vaccine (Fel-O-Vax Lv-K) displayed higher anti-SU antibody ELISA responses than a comparable group vaccinated with the polyvalent vaccine (Fel-O-Vax 5) ( < 0.001 for both anti-FeLV-A and FeLV-B SU antibody responses). This difference in anti-SU antibody responses between cats vaccinated with the monovalent or polyvalent vaccine, however, was not observed in cats that had been naturally exposed to FeLV (NAb-positive) ( = 0.33). It was postulated that vaccination with Fel-O-Vax 5 primed the humoral response prior to FeLV exposure, such that antibody production increased when the animal was challenged, while vaccination with Fel-O-Vax Lv-K induced an immediate preparatory antibody response that did not quantitatively increase after FeLV exposure. These results raise questions about the comparable vaccine efficacy of the different FeLV vaccine formulations and correlates of protection.

摘要

一项在澳大利亚进行的现场研究比较了两种已灭活的猫白血病病毒（FeLV）疫苗（单价疫苗 Fel-O-Vax Lv-K 和多价疫苗 Fel-O-Vax 5）在已接种疫苗的宠物猫中诱导的抗体反应。对来自 428 只未感染 FeLV 的猫（118 只接种过 FeLV 疫苗，310 只未接种过 FeLV 疫苗）的血清样本进行抗 FeLV 中和抗体（NAb）检测，使用活病毒中和测定法识别 378 只未暴露（NAb 阴性）和 50 只暴露（NAb 阳性；流产感染）的猫，随后进行抗表面单位（SU）FeLV-A 和 FeLV-B 抗体 ELISA 检测。另外还对 42 只 FeLV 感染猫（28 只假定退行性感染，14 只假定进行性感染）进行了抗 SU 抗体检测。与 NAb 阴性猫相比，NAb 阳性猫的抗 SU 抗体 ELISA 反应明显更高（<0.001）。与使用多价疫苗（Fel-O-Vax 5）接种的可比组相比，在先前接种单价疫苗（Fel-O-Vax Lv-K）后不到 18 个月接种疫苗的未暴露（NAb 阴性）猫的抗 SU 抗体 ELISA 反应更高（抗 FeLV-A 和 FeLV-B SU 抗体反应均 <0.001）。然而，在自然暴露于 FeLV 的猫（NAb 阳性）中，未观察到用单价或多价疫苗接种的猫之间的抗 SU 抗体反应差异（=0.33）。有人推测，Fel-O-Vax 5 疫苗接种在 FeLV 暴露前激发了体液反应，因此当动物受到挑战时，抗体产生增加，而 Fel-O-Vax Lv-K 疫苗接种则诱导了即时的预备性抗体反应，在 FeLV 暴露后不会定量增加。这些结果引发了对不同 FeLV 疫苗制剂的可比疫苗效力和保护相关性的质疑。