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体外筛选作为加利福虫（Calicophoron daubneyi）驱虫药物发现的一个途径：基于营养介质和瘤胃环境的方法。

In vitro screening as an anthelmintic discovery pipeline for Calicophoron daubneyi: nutritive media and rumen environment-based approaches.

机构信息

Institute of Biological, Environmental & Rural Sciences (IBERS), Aberystwyth University, Aberystwyth, SY23 3DA, UK.

Agri-Food and Biosciences Institute (AFBI), Large Park, Hillsborough, BT26 6DR, UK.

出版信息

Parasitol Res. 2021 Apr;120(4):1351-1362. doi: 10.1007/s00436-021-07066-2. Epub 2021 Feb 6.

DOI:10.1007/s00436-021-07066-2
PMID:33547506
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7940163/
Abstract

Paramphistomosis can lead to morbidity and mortality of ruminant livestock within tropical and sub-tropical climates. In recent decades, rumen fluke has become an emerging infection in temperate climates across Western Europe, with Calicophoron daubneyi, the primary species present. Clinical outbreaks with C. daubneyi larvae are reported and adults might be responsible for production losses. There is not currently a widely licensed anthelmintic product available to control C. daubneyi. In this study, three existing flukicide anthelmintics were tested for efficacy against mature C. daubneyi, comparing a standard in vitro culturing assay and a new more relevant rumen fluid based in vitro compound screening protocol. The new rumen based screen confirmed that oxyclozanide was active against adult C. daubneyi and identified activity with praziquantel. The study highlighted the downstream value of incorporating relevant in vitro screening for anthelmintic discovery pipelines.

摘要

片形吸虫病可导致热带和亚热带气候下反刍家畜的发病和死亡。近几十年来，双腔吸虫在西欧温带气候中成为一种新出现的感染，主要存在于加利科波龙属。据报道，有 C. daubneyi 幼虫引起的临床暴发，成虫可能导致生产损失。目前尚无广泛许可的驱虫药可用于控制 C. daubneyi。在这项研究中，测试了三种现有的驱虫药对成熟的 C. daubneyi 的疗效，比较了标准的体外培养测定法和一种新的更相关的基于瘤胃液的体外化合物筛选方案。新的基于瘤胃的筛选证实了奥克氯醛对成年 C. daubneyi 有效，并确定了吡喹酮的活性。该研究强调了在驱虫药发现管道中纳入相关体外筛选的下游价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/9be50db9c136/436_2021_7066_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/d6c751ef2c5f/436_2021_7066_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/48ec1f70b32f/436_2021_7066_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/91e9ab713e6c/436_2021_7066_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/f512286e92e2/436_2021_7066_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/8f4ed255b8da/436_2021_7066_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/9be50db9c136/436_2021_7066_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/d6c751ef2c5f/436_2021_7066_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/48ec1f70b32f/436_2021_7066_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/91e9ab713e6c/436_2021_7066_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/f512286e92e2/436_2021_7066_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/8f4ed255b8da/436_2021_7066_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2254/7940163/9be50db9c136/436_2021_7066_Fig6_HTML.jpg

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