State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
Mol Biol Rep. 2021 Feb;48(2):1269-1279. doi: 10.1007/s11033-021-06197-0. Epub 2021 Feb 5.
Auxin is an important endogenous hormone in plants. The YUCCA gene encodes a flavin monooxygenase, which is an important rate-limiting enzyme in the auxin synthesis pathway and involved in the regulation of plant growth and development. In the study, we identified 63 wheat TaYUCCA genes; among them, some genes appeared in clusters. By constructing phylogenetic trees, we found that the TaYUCCA genes could be divided into six groups. In the WheatExp database, there were 22 differential expressed TaYUCCA genes, among which the TaYUCCA10 gene was abundantly expressed in the endosperm and medium milk stage, the TaYUCCA2 gene was abundantly expressed in the roots of three leaves and meiosis and transfer cells at 20 days post anthesis and the others 16 TaYUCCA genes had different expression level at different developmental stages in wheat, and there were 15 TaYUCCA genes induced by drought and heat stress, among which the TaYUCCA2-D, TaYUCCA3-B, and TaYUCCA9-D might be upregulated induced by drought stress, TaYUCCA10.1 might be upregulated induced drought and heat stress, TaYUCCA6-A was upregulated induced both drought and heat stress and the others 9 TaYUCCA genes were downregulated induced by drought and heat stress. Transcriptome and qRT-PCR analysis showed that TaYUCCA7-A was upregulated significantly after induced by powdery mildew. The comprehensive annotation and expression profiling of the TaYUCCA genes in this study enhanced our understanding of TaYUCCA family gene expression in wheat growth and development and laid the foundation for the further study of TaYUCCA gene mechanism.
植物中的生长素是一种重要的内源性激素。YUCCA 基因编码黄素单加氧酶，它是生长素合成途径中的一个重要限速酶，参与植物生长发育的调节。在研究中，我们鉴定了 63 个小麦 TaYUCCA 基因；其中，一些基因出现在簇中。通过构建系统发育树，我们发现 TaYUCCA 基因可以分为六个组。在 WheatExp 数据库中，有 22 个差异表达的 TaYUCCA 基因，其中 TaYUCCA10 基因在胚乳和中乳期大量表达，TaYUCCA2 基因在三叶期和减数分裂及转细胞期大量表达，其他 16 个 TaYUCCA 基因在小麦不同发育阶段的表达水平不同，有 15 个 TaYUCCA 基因受到干旱和热胁迫的诱导，其中 TaYUCCA2-D、TaYUCCA3-B 和 TaYUCCA9-D 可能受到干旱胁迫的上调诱导，TaYUCCA10.1 可能受到干旱和热胁迫的上调诱导，TaYUCCA6-A 受到干旱和热胁迫的上调诱导，其他 9 个 TaYUCCA 基因受到干旱和热胁迫的下调诱导。转录组和 qRT-PCR 分析表明，TaYUCCA7-A 在受到白粉病诱导后显著上调。本研究中对 TaYUCCA 基因在小麦生长发育过程中的综合注释和表达谱分析，增强了我们对 TaYUCCA 家族基因在小麦生长发育过程中表达的理解，为进一步研究 TaYUCCA 基因机制奠定了基础。
