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[被动血凝法检测及滴定抗破伤风抗体]

[Detection and titration of anti-tetanus antibodies by passive hemagglutination].

作者信息

Benamon D, Garretta M, Muller A

出版信息

Rev Fr Transfus Immunohematol. 1977 Sep;20(3):467-72. doi: 10.1016/s0338-4535(77)80080-9.

Abstract

Human red cells O Rh + are coated with purified tetanus toxoid, by means of chromic chloride as coupling agent. With this reagent and by passive haemagglutination technique, antitetanus antibodies can be automatically screened and titrated in blood donnors plasmas, on Groupamatic equipments. Comparative results of three techniques: counter immunoelectrophoresis; manual passive haemagglutination; automatic passive haemagglutination are given, 4, 92 percent among the 1.219 tested blood donnors plasmas have an antibody greater than or equal to 5 U.I., 16, 24 percent have a titre between 1 and 5 U.I.

摘要

人类O型Rh阳性红细胞通过氯化铬作为偶联剂包被纯化破伤风类毒素。使用该试剂并通过被动血凝技术,可在Groupamatic设备上自动筛查和滴定献血者血浆中的抗破伤风抗体。给出了三种技术的比较结果:对流免疫电泳;手动被动血凝;自动被动血凝。在1219份检测的献血者血浆中,4.92%的血浆抗体大于或等于5国际单位,16.24%的血浆效价在1至5国际单位之间。

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