National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala, India.
Dept. of Marine Biology, Microbiology and Biochemistry, Cochin University of Science and Technology, Fine Arts Avenue, Kochi, Kerala, India.
Int J Biol Macromol. 2021 Apr 15;176:244-252. doi: 10.1016/j.ijbiomac.2021.02.006. Epub 2021 Feb 4.
Polyhydroxyalkanoates (PHAs) are natural polyesters produced by microorganisms as a source of intracellular energy reserves. These polymers have been extensively studied for tissue engineering and drug delivery applications due to their desirable material properties. Solvent-cast film of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), produced by Bacillus cereus MCCB 281 was characterized to study the surface morphology, roughness, thermal and mechanical properties. PHBV films were slightly hydrophilic with an average surface roughness of 43.66 nm. In vitro cell viability and proliferation studies on PHBV film surface investigated using L929 fibroblasts showed good cell attachment and proliferation. Hemocompatibility of PHBV evaluated by hemolysis assay, in vitro platelet adhesion and coagulation assays demonstrated good blood compatibility for use as blood contact graft materials. Therefore, PHBV produced from the marine bacterium favoured cellular growth of L929 fibroblasts indicating its potential to be used as a biomaterial substrate for cell adhesion in tissue engineering and medical applications.
聚羟基烷酸酯（PHAs）是微生物作为细胞内能量储备来源产生的天然聚酯。由于其理想的材料性能，这些聚合物已被广泛研究用于组织工程和药物输送应用。由蜡状芽孢杆菌 MCCB 281 生产的聚（3-羟基丁酸-co-3-羟基戊酸）（PHBV）的溶剂浇铸膜进行了表征，以研究其表面形貌、粗糙度、热性能和机械性能。PHBV 薄膜具有轻微的亲水性，平均表面粗糙度为 43.66nm。使用 L929 成纤维细胞对 PHBV 薄膜表面进行的体外细胞活力和增殖研究表明，细胞附着和增殖良好。通过溶血试验、体外血小板黏附试验和凝血试验评估 PHBV 的血液相容性表明其具有良好的血液相容性，可作为血液接触移植物材料。因此，海洋细菌产生的 PHBV 有利于 L929 成纤维细胞的生长，表明其有可能作为组织工程和医疗应用中细胞黏附的生物材料基质。
