Bahador Mason, Alfirdous Rayyan A, Alquria Theeb A, Griffin Ina L, Tordik Patricia A, Martinho Frederico C
Division of Endodontics, Department of Advanced Oral Sciences and Therapeutics, University of Maryland, School of Dentistry, Baltimore, Maryland.
Division of Endodontics, Department of Advanced Oral Sciences and Therapeutics, University of Maryland, School of Dentistry, Baltimore, Maryland; Prince Abdulrahman Advanced Dental Institute, Riyadh, Kingdom of Saudi Arabia.
J Endod. 2021 May;47(5):732-739. doi: 10.1016/j.joen.2021.01.009. Epub 2021 Feb 3.
The aims of this study were to investigate aerosolized microorganisms generated during endodontic emergencies and nonsurgical root canal therapy (NSRCT), to assess the spread of airborne microbes, and to verify the spatial distribution of airborne microbial spread.
A total of 45 endodontic procedures were sampled, including full pulpotomy (n = 15), pulpectomy (n = 15), and NSRCT (n = 15). Samples were collected during room resting and after treatment. The passive air sampling technique using settle plates was applied. Agar plates were set at different locations in the operatory. The colony-forming unit (CFU) was counted in brain-heart infusion blood agar plates. A set of agar plates containing selective chromogenic culture media was used for the isolation and presumptive identification of target microorganisms. Fungi were investigated using Sabouraud dextrose agar.
Pulpotomy generated the lowest mean CFU count (P < .05). There was no difference between the mean CFU counts found in pulpectomy and NSRCT (P > .05). A higher mean CFU count was found close to the patient's mouth (0.5 m) than at a 2-m distance in pulpectomy and NSRCT (P < .05). There was no difference between the mean CFU count found in front of the patient's mouth versus diagonal in pulpectomy and NSRCT (P > .05). Staphylococcus aureus (22/45, 48.8%) was the most frequent bacteria species. Longer treatment times were associated with higher CFU counts.
Our findings indicated that pulpotomy generates less aerosolized microorganisms than pulpectomy and NSRCT. The proximity to the patient's mouth and the treatment duration were implicated in the level of contamination.
本研究的目的是调查牙髓病急症和非手术根管治疗（NSRCT）过程中产生的雾化微生物，评估空气传播微生物的扩散情况，并验证空气传播微生物扩散的空间分布。
共对45例牙髓治疗操作进行了采样，包括全牙髓切断术（n = 15）、牙髓摘除术（n = 15）和非手术根管治疗（n = 15）。在诊室休息期间和治疗后采集样本。采用沉降平板的被动空气采样技术。琼脂平板放置在操作间的不同位置。在脑心浸液血琼脂平板上计数菌落形成单位（CFU）。使用一组含有选择性显色培养基的琼脂平板进行目标微生物的分离和初步鉴定。使用沙氏葡萄糖琼脂对真菌进行研究。
牙髓切断术产生的平均CFU计数最低（P < .05）。牙髓摘除术和非手术根管治疗的平均CFU计数之间没有差异（P > .05）。在牙髓摘除术和非手术根管治疗中，靠近患者口腔（0.5米）处的平均CFU计数高于2米处（P < .05）。在牙髓摘除术和非手术根管治疗中，患者口腔前方与对角位置的平均CFU计数之间没有差异（P > .05）。金黄色葡萄球菌（22/45，48.8%）是最常见的细菌种类。治疗时间越长，CFU计数越高。
我们的研究结果表明，牙髓切断术产生的雾化微生物比牙髓摘除术和非手术根管治疗少。靠近患者口腔的程度和治疗持续时间与污染程度有关。
