Key Laboratory of Meat Processing and Quality Control, MOE, China-US Joint Research Center for Food Safety and Quality, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology; Nanjing Agricultural University, Nanjing, 210095, PR China.
Key Laboratory of Meat Processing and Quality Control, MOE, China-US Joint Research Center for Food Safety and Quality, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology; Nanjing Agricultural University, Nanjing, 210095, PR China.
Microb Pathog. 2021 Apr;153:104776. doi: 10.1016/j.micpath.2021.104776. Epub 2021 Feb 3.
Listeria monocytogenes is a foodborne pathogen that causes systemic infections by crossing the intestinal barrier. However, in vitro analysis of the interaction of L. monocytogenes and small intestinal epithelium has yet to be fully elucidated. To study host responses from intestinal epithelium during L. monocytogenes infection, we used the co-culture model of small intestinal organoids and L. monocytogenes. Results showed that L. monocytogenes mediated damage to intestinal epithelium, especially intestinal stem cells. L. monocytogenes was found to reduce budding rate and increase mortality of organoids. Moreover, it affected the proliferation of epithelial cells and numbers of secretory cells. In addition, it was demonstrated that L. monocytogenes stimulated a reduction in the number of Lgr5+ stem cells. Furthermore, L. monocytogenes affected the expression of Hes1, Math1 and Sox9 to interfere with the differentiation of intestinal stem cells. Collectively, our findings reveal the effects of L. monocytogenes infection on intestinal stem cells and demonstrate that small intestinal organoid is a suitable experimental model for studying intestinal epithelium-pathogen interactions.
李斯特菌是一种食源性病原体，通过穿过肠道屏障引起全身感染。然而，李斯特菌与小肠上皮细胞相互作用的体外分析尚未完全阐明。为了研究宿主对李斯特菌感染的肠道上皮反应，我们使用了小肠类器官和李斯特菌的共培养模型。结果表明，李斯特菌介导了对肠道上皮细胞，特别是肠道干细胞的损伤。李斯特菌被发现降低了类器官的出芽率并增加了其死亡率。此外，它还影响了上皮细胞的增殖和分泌细胞的数量。此外，已经证明李斯特菌刺激了 Lgr5+干细胞数量的减少。此外，李斯特菌影响 Hes1、Math1 和 Sox9 的表达，干扰肠道干细胞的分化。总之，我们的研究结果揭示了李斯特菌感染对肠道干细胞的影响，并表明小肠类器官是研究肠道上皮-病原体相互作用的合适实验模型。
