National Centre of Meat Quality and Safety Control, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing, China.
Front Cell Infect Microbiol. 2022 Mar 25;12:793335. doi: 10.3389/fcimb.2022.793335. eCollection 2022.
, as a model organism, is a causative agent of enteric pathogen that causes systemic infection. However, the interaction of and small intestinal epithelium has not been fully elucidated yet. In this study, mice and intestinal organoids were chosen as the models to investigate the influence of infection on the intestinal secretory cells and its differentiation-related pathways. Results confirmed the phenomenon of intestinal damage that infection could lead to villi damage in mice, which was accompanied by the increase of TNF-α production in jejunum as well as lipopolysaccharide (LPS) secretion in serum. Moreover, it was demonstrated that infection increased the number of goblet and Paneth cells in mice and intestinal organoids and upregulated the expression of and . Furthermore, decreased the relative expression of Notch pathway-related genes (, , , and ) while upregulating the relative expression of gene in mice and intestinal organoids. This indicated that infection caused the inhibition of Notch pathway, which may be the reason for the increased number of goblet and Paneth cells in the intestine. Collectively, these results are expected to provide more information on the mechanism of s infection in the intestine.
作为一种模式生物，是一种肠道病原体的病原体，可引起全身感染。然而，与小肠上皮细胞的相互作用尚未完全阐明。在这项研究中，选择小鼠和肠类器官作为模型，以研究感染对肠道分泌细胞及其分化相关途径的影响。结果证实了感染会导致小鼠肠损伤的现象，这伴随着回肠中 TNF-α产生的增加以及血清中脂多糖（LPS）的分泌。此外，研究表明感染增加了小鼠和肠类器官中杯状细胞和潘氏细胞的数量，并上调了和的表达。此外，感染降低了 Notch 通路相关基因（、、、和）的相对表达，同时上调了小鼠和肠类器官中基因的相对表达。这表明感染引起了 Notch 通路的抑制，这可能是肠道中杯状细胞和潘氏细胞数量增加的原因。总之，这些结果有望为肠道感染的机制提供更多信息。
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