Clower B R, Yoshioka J, Honma Y, Smith R R
Department of Anatomy, University of Mississippi Medical Center, Jackson 39216-4505.
Anat Rec. 1988 Feb;220(2):161-70. doi: 10.1002/ar.1092200207.
The role of blood platelets in producing early intimal changes in cerebral arteries following subarachnoid hemorrhage (SAH) was examined by using 18 cats. Experimental SAH was produced by a rupture of the proximal portion of the right middle cerebral artery. Following SAH, the scanning electron microscope revealed that structural alterations in the intimal layer of major cerebral arteries occurred as early as 2 hours and became more severe by 48 hours. Vascular alterations, which were predominantly detected in the ruptured vessel, consisted of endothelial cell corrugation, detachment, crater formation, intimal adhesion of platelets and red blood cells, intimal thrombi, and reendothelialization. When cats were pretreated prior to SAH with an anti-platelet-aggregating agent, OKY-1581, the intimal blood elements and thrombi were clearly reduced, and reendothelialization was not observed. However, endothelial cell changes in the OKY-1581-treated group were very similar to those occurring in the nontreated group. While these results suggest that bioactive substances contained within blood platelets, such as growth factors, serotonin, and norepinephrine, have little effect on producing endothelial cell injury, platelets may be important in the initiation of reendothelialization following vessel injury.
通过使用18只猫，研究了血小板在蛛网膜下腔出血（SAH）后大脑动脉早期内膜变化中的作用。实验性SAH通过右大脑中动脉近端破裂产生。SAH后，扫描电子显微镜显示大脑主要动脉内膜层的结构改变早在2小时就出现，并在48小时时变得更加严重。血管改变主要在破裂血管中检测到，包括内皮细胞皱缩、脱离、坑洼形成、血小板和红细胞的内膜粘附、内膜血栓形成以及再内皮化。当猫在SAH前用抗血小板聚集剂OKY-1581预处理时，内膜血液成分和血栓明显减少，且未观察到再内皮化。然而，OKY-1581治疗组的内皮细胞变化与未治疗组非常相似。虽然这些结果表明血小板中所含的生物活性物质，如生长因子、5-羟色胺和去甲肾上腺素，对产生内皮细胞损伤影响很小，但血小板可能在血管损伤后的再内皮化起始中起重要作用。
