Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing 210098, PR China.
Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing 210098, PR China.
Bioresour Technol. 2021 Apr;326:124727. doi: 10.1016/j.biortech.2021.124727. Epub 2021 Jan 19.
This study explored biofloc technology for shrimp culture based on straw substrates with a size of 40 mu, 80 mu, and 120 mu. Straw substrates utilization stimulated shrimp growth compared to control. Treatment with 40 mu had the best ammonium (71.60%) and nitrite nitrogen (77.78%) removal rates generally. In all biofloc treatments, Proteobacteria (4.10-56.1%) was the most dominant phylum, followed by Bacteroidetes (2.44-38.21%), Planctomycetes (0.45-21.41%), and Verrucomicrobia (1.2-10.30%). Redundancy analysis showed that salinity was a significant factor closely related to the microbial community in biofloc. The environmental parameters (DO > pH > TN > NH-N > COD > Salinity > EC), nitrification, and denitrification genes (amoA > napA > nirK) were significant factors that interrelated with the bacterial genus in the network analysis. This study highlighted a novel technology of reusing agricultural waste that transformed inorganic nitrogen using nutrient recycling to control water quality in the culture system and produced microbial proteins that served as a natural nutritional supplement to enhance shrimp growth.
本研究探索了基于 40µm、80µm 和 120µm 稻草基质的生物絮团技术在虾养殖中的应用。与对照组相比，稻草基质的利用刺激了虾的生长。处理 40µm 的稻草时，氨氮（71.60%）和亚硝酸盐氮（77.78%）的去除率最高。在所有生物絮团处理中，变形菌门（4.10-56.1%）是最主要的门，其次是拟杆菌门（2.44-38.21%）、浮霉菌门（0.45-21.41%）和疣微菌门（1.2-10.30%）。冗余分析表明，盐度是与生物絮团中微生物群落密切相关的重要因素。环境参数（DO>pH>TN>NH-N>COD>Salinity>EC）、硝化和反硝化基因（amoA>napA>nirK）是与网络分析中细菌属相互关联的重要因素。本研究强调了一种利用农业废弃物的新型技术，该技术通过养分循环将无机氮转化为生物可利用氮，从而控制养殖系统中的水质，并产生微生物蛋白作为天然营养补充剂来促进虾的生长。
