Huang Jin, Li Jinghao, Zhou Wenzong, Cheng Yongxu, Li Jiayao
Key Laboratory of Integrated Rice-Fish Farming Ecosystem, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China.
Centre for Research on Environmental Ecology and Fish Nutrition (CREEFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai, China.
Front Microbiol. 2023 Aug 10;14:1233815. doi: 10.3389/fmicb.2023.1233815. eCollection 2023.
Although the microbial ecology of integrated rice-crayfish farming systems is receiving increasing attention with the expanding application area in China, the effects of rice transplanting patterns on the microbial community of water, sediment and intestine in rice-crayfish system has yet to be determined. This study explored the microbial community present in water, sediment and intestine samples from three transplant patterns (rice crayfish with wide-narrow row transplanting, rice-crayfish with normal transplanting and pond-crayfish, abbreviated as RC-W, RC, and PC, respectively) using high-throughput sequencing. The results showed that the dominant microbial taxa from sediment, surrounding water, and intestine at phylum level were Proteobacteria, Chloroflexi, Cyanobacteria, Actinobacteria, Bacteroidetes. The patterns of rice transplanting had significant effects on microbial biodiversity and species composition in surrounding water. The OTUs community richness of water under RC group was significantly higher than that of PC group and RC-W group. The OTU relative abundance of top 10 operational taxonomic units had significantly different ( < 0.05) in the water samples from the three groups. The intestinal OTU community richness of in the three groups was positively correlated with the community richness of water. The proximity between intestinal and water samples in PCA diagram indicated that their species composition was more similar. The results also showed that rice transplanting patterns can affect intestinal microbial biodiversity of and the intestinal microbial biodiversity correlated with water bodies. Although the intestinal microbial diversity of crayfish in RC-W group was lower than that in RC group, the relative abundance of potential pathogenic bacteria, such as , in intestine of the crayfish in the RC-W group was significantly decreased under rice wide-narrow row transplanting model. Redundancy analysis revealed that environmental parameters, such as pH, DO, nitrate, which regulate the composition of microbial community structures. This study provides an understanding for microbial response to different rice transplanting pattern in rice-crayfish farming system.
尽管随着稻虾综合种养系统在中国的应用面积不断扩大，其微生物生态受到了越来越多的关注，但水稻移栽模式对稻虾系统中水、沉积物和肠道微生物群落的影响尚未明确。本研究采用高通量测序技术，探究了三种移栽模式（宽窄行稻虾、常规稻虾和池塘养虾，分别简称为RC-W、RC和PC）的水、沉积物和肠道样本中的微生物群落。结果表明，在门水平上，沉积物、周围水体和肠道中的优势微生物类群为变形菌门、绿弯菌门、蓝细菌门、放线菌门、拟杆菌门。水稻移栽模式对周围水体中的微生物多样性和物种组成有显著影响。RC组水体的OTU群落丰富度显著高于PC组和RC-W组。三组水样中前10个操作分类单元的OTU相对丰度存在显著差异（<0.05）。三组中肠道的OTU群落丰富度与水体的群落丰富度呈正相关。主成分分析图中肠道样本与水体样本的接近程度表明它们的物种组成更为相似。结果还表明，水稻移栽模式可影响小龙虾肠道微生物多样性，且肠道微生物多样性与水体相关。虽然RC-W组小龙虾的肠道微生物多样性低于RC组，但在宽窄行水稻移栽模式下，RC-W组小龙虾肠道中潜在病原菌如[具体菌名未给出]的相对丰度显著降低。冗余分析表明，pH、溶解氧、硝酸盐等环境参数调节着微生物群落结构的组成。本研究为理解稻虾养殖系统中微生物对不同水稻移栽模式的响应提供了依据。
