Department of Orthodontics, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, China.
Department of Orthodontics, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, China.
Am J Orthod Dentofacial Orthop. 2021 Apr;159(4):415-425.e1. doi: 10.1016/j.ajodo.2020.01.025. Epub 2021 Feb 3.
Identification of pubertal growth peak is of great importance for the orthopedic treatment of Class II malocclusion. Our previous work demonstrated that vitamin D binding protein (DBP) and serotransferrin (TF) in gingival crevicular fluid (GCF) could be candidate biomarkers of pubertal growth peak. This research aimed to preliminarily validate TF and DBP in subjects with Class I and Class II malocclusion, to compare their diagnostic accuracy, and to construct a statistic model to help the diagnosis of skeletal pubertal peak.
Sixty-six circumpubertal subjects were recruited, including 32 subjects with Class I malocclusion and 34 subjects with Class II malocclusion. All subjects were divided into prepubertal, pubertal, and postpubertal groups according to their cervical vertebral maturation stages. GCF samples were collected, and the concentration of DBP and TF were detected by enzyme-linked immunosorbent assay.
Percentage of TF in GCF was significantly higher in pubertal than in prepubertal and postpubertal groups, in subjects with Class I and Class II malocclusion, whereas the difference observed in DBP was less significant. The diagnostic accuracy of TF was better than DBP and chronological age. The most optimal thresholds of maxillary and mandibular TF in distinguishing pubertal from nonpubertal subjects were 4.20% and 4.09%, respectively. The combination of TF and age exhibited the best diagnostic accuracy.
TF in GCF could be considered as a potential biomarker of pubertal peak and can assist the diagnosis of skeletal pubertal peak.
确定青春期生长高峰对于 II 类错[牙合]的矫形治疗非常重要。我们之前的研究表明，牙龈沟液（GCF）中的维生素 D 结合蛋白（DBP）和转铁蛋白（TF）可能是青春期生长高峰的候选生物标志物。本研究旨在初步验证 TF 和 DBP 在 I 类和 II 类错[牙合]患者中的作用，比较它们的诊断准确性，并构建一个统计模型以帮助诊断骨骼青春期高峰。
招募了 66 名青春期前的受试者，包括 32 名 I 类错[牙合]患者和 34 名 II 类错[牙合]患者。所有受试者均根据颈椎成熟度分为青春前期、青春期和青春后期组。采集 GCF 样本，采用酶联免疫吸附试验检测 DBP 和 TF 的浓度。
在 I 类和 II 类错[牙合]患者中，TF 在 GCF 中的百分比在青春期明显高于青春前期和青春后期，而 DBP 的差异则不那么显著。TF 的诊断准确性优于 DBP 和年龄。区分青春期和非青春期受试者的上颌和下颌 TF 的最佳阈值分别为 4.20%和 4.09%。TF 和年龄的组合具有最佳的诊断准确性。
GCF 中的 TF 可作为青春期高峰的潜在生物标志物，有助于骨骼青春期高峰的诊断。
