Nutrition and Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Student Research Committee, Department of Clinical Nutrition and Dietetics, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Nutrition and Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Nutr Metab Cardiovasc Dis. 2021 Mar 10;31(3):894-901. doi: 10.1016/j.numecd.2020.11.019. Epub 2020 Nov 27.
Omentin, as an adipokine, has been reported to improve insulin resistance and inflammation may be related to fatty acids (FAs). Plasma FAs can be used as biomarkers of dietary FAs and endogenous FA exposure. We aimed to evaluate the association between plasma FAs pattern and omentin gene expression in adipose tissue (AT).
Visceral and subcutaneous AT and fasting blood were gathered from 97 adults aged >18 years. Participants were already admitted to hospitals for elective abdominal surgery. Dietary intakes were assessed using a food frequency questionnaire. The relative omentin gene expression in visceral and subcutaneous AT was measured by Real-Time PCR and plasma FAs was determined by gas chromatography. The principal component analysis was performed to derive the FAs pattern from plasma individual FAs. Three patterns were derived from plasma FAs, 1) high de-novo lipogenesis (DNL), 2) high trans saturated fatty acids (SFA), and docosahexaenoic acid (trans-SFA/DHA), and 3) high long-chain SFA (LC-SFA). After adjustment for age, sex, and insulin concentration, only the LC-SFA pattern was associated with omentin gene expression in visceral AT (β = 2.25, P = 0.03). Other patterns were not associated with omentin gene expression in visceral and subcutaneous AT.
A pattern characterized by high levels of myristic acid (14:0), heptadecanoic acid (17:0), pentadecanoic acid (15:0), and Cis_heptadecanoic acid (17:1), which named LC-SFA was related to omentin gene expression in visceral AT.
作为一种脂肪因子，内脂素被报道可以改善胰岛素抵抗，其炎症可能与脂肪酸（FAs）有关。血浆 FAs 可以作为膳食 FAs 和内源性 FA 暴露的生物标志物。我们旨在评估血浆 FAs 谱与脂肪组织（AT）中内脂素基因表达之间的关系。
从 97 名年龄>18 岁的成年人中采集内脏和皮下 AT 及空腹血。参与者已经因择期腹部手术而住院。使用食物频率问卷评估膳食摄入量。通过实时 PCR 测量内脏和皮下 AT 中的相对内脂素基因表达，并通过气相色谱法测定血浆 FAs。通过主成分分析从血浆个体 FAs 中得出 FAs 谱。从血浆 FAs 中得出了三种模式，1）高从头合成（DNL），2）高反式饱和脂肪酸（SFA）和二十二碳六烯酸（反式 SFA/DHA），3）高长链 SFA（LC-SFA）。调整年龄、性别和胰岛素浓度后，只有 LC-SFA 模式与内脏 AT 中内脂素基因表达相关（β=2.25，P=0.03）。其他模式与内脏和皮下 AT 中的内脂素基因表达无关。
一种以肉豆蔻酸（14:0）、十七烷酸（17:0）、十五烷酸（15:0）和顺式十七烷酸（17:1）水平较高为特征的模式，命名为 LC-SFA，与内脏 AT 中内脂素基因表达相关。
