Cellular and Molecular Endocrine Research Center (CMERC), Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, PO Box: 19395-4763, Tehran, Iran.
Nutrition and Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mol Biol Rep. 2022 Jan;49(1):163-169. doi: 10.1007/s11033-021-06854-4. Epub 2021 Nov 5.
Adipose tissue (AT) is a passive reservoir for energy storage and an active endocrine organ responsible for synthesizing bioactive molecules called adipokines. Omentin is known as an anti-inflammatory adipokine that can modulate insulin sensitivity. The present study aimed to investigate the relationship between omentin mRNA expression and glucose homeostasis of visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) in non-diabetic adults.
VAT and SAT adipose tissues were collected from 137 adults aged ≥ 18 years hospitalized for abdominal surgery. Before surgery, preoperative blood samples were taken from the participants to measure fasting plasma glucose, insulin, and triglyceride. BMI, HOMA-IR, HOMA-B, and QUICKI were calculated. Insulin levels were measured with Mercodia kits using enzyme-linked immunosorbent assay (ELISA). In order to obtain omentin mRNA expression, real-time PCR was performed.
Overall, 91 (66.4%) subjects were healthy [without insulin resistance (IR)], and 46 (33.6%) participants were with IR. In healthy and IR subjects, omentin gene expression was 1.04 and 2.32, respectively in VAT, and 3.06 and 1.30, respectively, in SAT (P > 0.05). After controlling for age and BMI, linear regression analysis indicated a significant positive association of SAT omentin expression with insulin concentration (β = 0.048; 95% CI 0.009, 0.088, P = 0.017) and HOMA-IR (β = 0.173; 95% CI 0.023, 0.323, P = 0.014). Moreover, a negative association of SAT omentin expression with HOMA-B (β = - 0.001; 95% CI 0.002, - 0.001, P < 0.001) was observed.
This study's finding confirms a direct association between IR with omentin mRNA levels in SAT. Besides, the indicator of insulin sensitivity had an inverse association with omentin gene expression in SAT. This aspect of research suggests that omentin secretion from SAT has a strong link with insulin regulation.
脂肪组织（AT）是能量储存的被动库，也是一种活跃的内分泌器官，负责合成称为脂肪因子的生物活性分子。内脂素是一种具有抗炎作用的脂肪因子，可调节胰岛素敏感性。本研究旨在探讨非糖尿病成年人内脏脂肪组织（VAT）和皮下脂肪组织（SAT）中内脂素 mRNA 表达与葡萄糖稳态之间的关系。
从 137 名年龄≥18 岁因腹部手术住院的成年人中采集 VAT 和 SAT 脂肪组织。手术前，从参与者中抽取术前血样，测量空腹血糖、胰岛素和甘油三酯。计算 BMI、HOMA-IR、HOMA-B 和 QUICKI。使用酶联免疫吸附测定法（ELISA）用 Mercodia 试剂盒测量胰岛素水平。为了获得内脂素 mRNA 表达，进行实时 PCR。
总体而言，91 名（66.4%）受试者健康[无胰岛素抵抗（IR）]，46 名（33.6%）参与者存在 IR。在健康和 IR 受试者中，VAT 中的内脂素基因表达分别为 1.04 和 2.32，SAT 中分别为 3.06 和 1.30（P>0.05）。在控制年龄和 BMI 后，线性回归分析表明 SAT 内脂素表达与胰岛素浓度呈显著正相关（β=0.048；95%CI0.009，0.088，P=0.017）和 HOMA-IR（β=0.173；95%CI0.023，0.323，P=0.014）。此外，还观察到 SAT 内脂素表达与 HOMA-B 呈负相关（β=-0.001；95%CI0.002，-0.001，P<0.001）。
本研究的发现证实了 SAT 中 IR 与内脂素 mRNA 水平之间存在直接关联。此外，胰岛素敏感性指标与 SAT 中内脂素基因表达呈负相关。这方面的研究表明，SAT 中内脂素的分泌与胰岛素调节有很强的联系。
