SCASP：一种简单而稳健的 SDS 辅助蛋白质组学研究样品制备方法。
SCASP: A Simple and Robust SDS-Aided Sample Preparation Method for Proteomic Research.
机构信息
State Key Laboratory of Cellular Stress Biology, Innovation Center for Cellular Signaling Network, School of Life Sciences, Xiamen University, Xiamen, China.
Department of Emergency, Zhongshan Hospital, Xiamen University, Xiamen, China.
出版信息
Mol Cell Proteomics. 2021;20:100051. doi: 10.1016/j.mcpro.2021.100051. Epub 2021 Feb 5.
SDS is widely used in sample preparation for proteomic research. However, SDS is incompatible with LC and electrospray ionization. SDS depletion is therefore required ahead of LC-MS analysis. Most of current SDS removal strategies are time consuming, laborious, and have low reproducibility. Here, we describe a method, SDS-cyclodextrin (CD)-assisted sample preparation, by which CD can bind to SDS and form CD-SDS complexes in solutions, allowing for direct tryptic digestion. We demonstrate that SDS-CD-assisted sample preparation is a simple, fast, and robust SDS-based sample preparation method for proteomics application.
SDS 在蛋白质组学研究的样品制备中被广泛应用。然而，SDS 与 LC 和电喷雾离子化不兼容。因此，在 LC-MS 分析之前需要进行 SDS 耗尽。目前大多数 SDS 去除策略都耗时、费力且重复性低。在这里，我们描述了一种 SDS-环糊精 (CD)-辅助样品制备方法，其中 CD 可以与 SDS 结合形成溶液中的 CD-SDS 复合物，从而可以直接进行胰蛋白酶消化。我们证明 SDS-CD 辅助样品制备是一种简单、快速且稳健的基于 SDS 的蛋白质组学应用样品制备方法。
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