Li Dak Sum Yip Yio Chin R & D Center for Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
Li Dak Sum Yip Yio Chin R & D Center for Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China; State Key Laboratory of Research on Bioactivities and Clinical Application of Medicinal Plants, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China.
J Pharm Biomed Anal. 2021 Mar 20;196:113920. doi: 10.1016/j.jpba.2021.113920. Epub 2021 Jan 26.
DNA barcoding is a widely used tool for species identification and authentication. However, it may not be applicable to highly processed herbal products due to severe DNA fragmentation. The emergence of DNA metabarcoding provides an alternative way to solve the problem. In this study, we are the first to combine the use of adaptor ligation-mediated PCR method and metabarcoding to reveal species identities in herbal products. As an illustration, we applied the method on three Hedyotis herbal products collected from China and Thailand. Results showed that H. diffusa and H. corymbosa were present in the products which were consistent with their label claims. Our study indicated that the adaptor ligation-mediated PCR with metabarcoding approach is useful for authentication of highly processed herbal products.
DNA 条形码是一种广泛用于物种鉴定和认证的工具。然而，由于 DNA 严重碎片化，它可能不适用于高度加工的草药产品。DNA 代谢组学的出现提供了一种解决该问题的替代方法。在这项研究中，我们首次将接头连接介导的 PCR 方法和代谢组学结合起来，以揭示草药产品中的物种身份。作为说明，我们将该方法应用于从中国和泰国收集的三种白花蛇舌草草药产品。结果表明，产品中存在白花蛇舌草和蛇舌草，这与它们的标签声称一致。我们的研究表明，接头连接介导的 PCR 与代谢组学方法相结合，可用于高度加工的草药产品的鉴定。
