Natural History Museum, University of Oslo, P.O. Box 1172, Blindern, 0318, Oslo, Norway.
Stejarul Research Centre for Biological Sciences, National Institute of Research and Development for Biological Sciences, Alexandru cel Bun Street, 6, 610004, Piatra Neamt, Romania.
Phytochem Anal. 2018 Mar;29(2):123-128. doi: 10.1002/pca.2732. Epub 2017 Sep 14.
Herbal medicines play an important role globally in the health care sector and in industrialised countries they are often considered as an alternative to mono-substance medicines. Current quality and authentication assessment methods rely mainly on morphology and analytical phytochemistry-based methods detailed in pharmacopoeias. Herbal products however are often highly processed with numerous ingredients, and even if these analytical methods are accurate for quality control of specific lead or marker compounds, they are of limited suitability for the authentication of biological ingredients.
To review the benefits and limitations of DNA barcoding and metabarcoding in complementing current herbal product authentication.
Recent literature relating to DNA based authentication of medicinal plants, herbal medicines and products are summarised to provide a basic understanding of how DNA barcoding and metabarcoding can be applied to this field.
Different methods of quality control and authentication have varying resolution and usefulness along the value chain of these products. DNA barcoding can be used for authenticating products based on single herbal ingredients and DNA metabarcoding for assessment of species diversity in processed products, and both methods should be used in combination with appropriate hyphenated chemical methods for quality control.
DNA barcoding and metabarcoding have potential in the context of quality control of both well and poorly regulated supply systems. Standardisation of protocols for DNA barcoding and DNA sequence-based identification are necessary before DNA-based biological methods can be implemented as routine analytical approaches and approved by the competent authorities for use in regulated procedures. © 2017 The Authors. Phytochemical Analysis Published by John Wiley & Sons Ltd.
草药在全球医疗保健领域发挥着重要作用，在工业化国家，它们通常被视为单一组分药物的替代品。当前的质量和鉴定评估方法主要依赖于药典中详述的形态和基于分析植物化学的方法。然而，草药产品通常经过高度加工，含有众多成分，即使这些分析方法对于特定先导或标记化合物的质量控制非常准确，但对于生物成分的鉴定，它们的适用性有限。
综述 DNA 条形码和代谢组学在补充当前草药产品鉴定方面的优势和局限性。
总结了与药用植物、草药和产品的 DNA 鉴定相关的最新文献，以便基本了解 DNA 条形码和代谢组学如何应用于该领域。
不同的质量控制和鉴定方法在这些产品的价值链上具有不同的分辨率和实用性。DNA 条形码可用于基于单一草药成分的产品鉴定，而 DNA 代谢组学可用于评估加工产品中的物种多样性，这两种方法都应与适当的化学联用方法结合使用，以进行质量控制。
DNA 条形码和代谢组学在监管良好和监管不善的供应系统的质量控制方面具有潜力。在将基于 DNA 的生物学方法作为常规分析方法实施并获得主管当局批准用于受监管程序之前，有必要对 DNA 条形码和基于 DNA 序列的鉴定协议进行标准化。© 2017 作者。植物化学分析 由 John Wiley & Sons Ltd 出版。
