School of Civil Engineering, Heilongjiang University, Harbin 150080, China.
School of Life Science, Heilongjiang University, Harbin 150080, China.
Bioresour Technol. 2021 Apr;326:124794. doi: 10.1016/j.biortech.2021.124794. Epub 2021 Feb 2.
For solving the challenge in nitrate removal from low C/N wastewater at low temperature, Pseudomonas sp. Y39-6 was isolated and used in nitrate removal. It showed aerobic-heterotrophic denitrification with rate of 1.77 ± 0.31 mg/L·h and unusual aerobic-autotrophic nitrate removal (rate of 0.324 mg/L·h). The aerobic-autotrophic nitrate removal mechanisms were deep investigated by analyzing the nitrate removal process and genomic information. At aerobic-autotrophic condition, the strain Y39-6 could assimilate nitrate to amino acid (NO + PHA + CO → CHON) with the carbon source from Polyhydroxyalkanoic acid (PHA) degradation and CO fixation. Flagella motivation, swarming activity and extracellular polymeric substances (EPS) production regulated Pseudomonas sp. Y39-6 forming biofilm. Carriers immobilized with Pseudomonas sp. Y39-6 were used in moving bed biofilm reactor (MBBR) and achieved 24.83% nitrate removal at C/N < 1 and 4 °C. Results of this study provided a practical way for nitrogen removal from low C/N wastewater in cold region.
为了解决低温低 C/N 废水中硝酸盐去除的难题，分离出了 Pseudomonas sp. Y39-6 并用于硝酸盐去除。它表现出好氧异养反硝化，硝酸盐去除速率为 1.77 ± 0.31 mg/L·h，且具有不寻常的好氧自养硝酸盐去除（速率为 0.324 mg/L·h）。通过分析硝酸盐去除过程和基因组信息，深入研究了好氧自养硝酸盐去除的机制。在好氧自养条件下，该菌株 Y39-6 可以将硝酸盐同化到氨基酸中（NO + PHA + CO → CHON），同时利用聚羟基烷酸（PHA）降解和 CO 固定作为碳源。鞭毛运动、群集活性和细胞外聚合物（EPS）的产生调节了 Pseudomonas sp. Y39-6 形成生物膜。用 Pseudomonas sp. Y39-6 固定化的载体用于移动床生物膜反应器（MBBR）中，在 C/N < 1 和 4°C 的条件下实现了 24.83%的硝酸盐去除率。本研究结果为寒冷地区低 C/N 废水的脱氮提供了一种实用的方法。
