Degirmentepe Recep B, Altunrende Fatih, Bozkurt Muammer, Merder Erkan, Otunctemur Alper, Sonmez Kivilcim, Yildirim Funda, Ada Saniye, Isman Ferruh K, Cekmen Mustafa B
Department of Urology, Of State Hospital, Trabzon, Turkey.
Department of Urology, University of Health Sciences Okmeydani Training and Research Hospital, Istanbul, Turkey.
Andrologia. 2021 May;53(4):e14000. doi: 10.1111/and.14000. Epub 2021 Feb 6.
This study was performed to evaluate the effect of liraglutide on experimental testicular ischaemia reperfusion in rats in terms of biochemistry, histopathology and immunohistochemistry. A total of 28 male Wistar-Albino rats were divided randomly into 4 groups: control (7), sham (7), ischaemia-reperfusion (7) and ischaemia-reperfusion + liraglutide (7). Biochemically, Nitric Oxide, Malondialdehyde, Superoxide dismutase, Glutathione peroxidase and Catalase levels were measured in the testis. Apoptosis protease activating factor-1 and inducible nitric oxide synthase activity were evaluated immunohistochemically as well. Statistical analyses were made via the Kruskal-Wallis and Mann-Whitney U tests. In the reperfusion group, CAT and SOD values were increased (p > .05), NO and MDA values were decreased (p < .05) after administration of liraglutide. In addition, GPx values were significantly increased in ischaemia reperfusion + liraglutide administered group compared to reperfusion group (p < .05). Apaf-1 and iNOS activity were significantly decreased with the addition of liraglutide treatment to the ischaemia-reperfusion group (p < .05). First of all, we would like to say that liraglutide treatment is moderately preventive against I/R injury in testicular torsion. The anti-inflammatory, antioxidant and antiapoptotic properties of liraglutide are create a moderately protective effect as we show in this study.
本研究旨在从生物化学、组织病理学和免疫组织化学方面评估利拉鲁肽对大鼠实验性睾丸缺血再灌注的影响。总共28只雄性Wistar - 白化大鼠被随机分为4组：对照组（7只）、假手术组（7只）、缺血再灌注组（7只）和缺血再灌注 + 利拉鲁肽组（7只）。在生物化学方面，检测了睾丸中的一氧化氮、丙二醛、超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶水平。还通过免疫组织化学评估了凋亡蛋白酶激活因子 - 1和诱导型一氧化氮合酶活性。通过Kruskal - Wallis和Mann - Whitney U检验进行统计分析。在再灌注组中，给予利拉鲁肽后，CAT和SOD值升高（p > 0.05），NO和MDA值降低（p < 0.05）。此外，与再灌注组相比，缺血再灌注 + 利拉鲁肽给药组的GPx值显著升高（p < 0.05）。在缺血再灌注组中加入利拉鲁肽治疗后，Apaf - 1和iNOS活性显著降低（p < 0.05）。首先，我们想说利拉鲁肽治疗对睾丸扭转中的缺血/再灌注损伤有一定的预防作用。正如我们在本研究中所示，利拉鲁肽的抗炎、抗氧化和抗凋亡特性产生了一定的保护作用。
