Bai Guo-Liang, Hu Kai-Li, Huan Yi, Wang Xing, Lei Lei, Zhang Meng, Guo Chun-Yan, Chang Hong-Sheng, Zhao Li-Bo, Liu Jing, Shen Zhu-Fang, Wang Xiao-Ling, Ni Xin
Clinical Research Center, Beijing Children's Hospital, National Center for Children's Health, Capital Medical University, Beijing, China.
State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Key Laboratory of Polymorphic Drugs of Beijing, Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Front Pharmacol. 2021 Jan 21;11:596525. doi: 10.3389/fphar.2020.596525. eCollection 2020.
The purpose of this study was to explore the effect of the traditional Chinese medicine Fuyou formula on precocious puberty (PP). The Fy formula may exert an effect in female rats with PP and GT-7 cells through the GPR54/GnRH signaling pathway. To confirm the effect of the Fy formula on PP through the GPR54/GnRH signaling pathway, we first treated GT1-7 cells with the Fy formula and observed changes in the expression of related genes and proteins and in GnRH secretion. Then, we randomly divided young female Sprague-Dawley rats into the control group, model group, leuprorelin group and the Fy formula group. A PP model was established by injection of danazol on postnatal day 5, and the Fy formula was administered on PND15. The time of vaginal opening, the wet weights of the ovary and uterus, serum hormone levels and the expression of hypothalamic-related genes were observed. We found that the Fy formula delayed vaginal opening, decreased the wet weights and coefficients of the ovary and uterus, decreased the levels of serum hormones (E2, follicle-stimulating hormone and luteinizing hormone) and the cellular GnRH level, and downregulated the gene expression of Kiss1, GPR54 and GnRH in the hypothalamus and the gene and protein expression of GPR54 and GnRH in GT1-7 cells. In conclusion, the Fy formula may alleviate PP via the GPR54/GnRH signaling pathway.
本研究旨在探讨中药复方福幼方对性早熟（PP）的影响。福幼方可能通过GPR54/促性腺激素释放激素（GnRH）信号通路对性早熟雌性大鼠及GT-7细胞发挥作用。为证实福幼方通过GPR54/GnRH信号通路对性早熟的影响，我们首先用福幼方处理GT1-7细胞，观察相关基因和蛋白表达及GnRH分泌的变化。然后，将幼年雌性斯普拉格-道利大鼠随机分为对照组、模型组、亮丙瑞林组和福幼方组。于出生后第5天注射达那唑建立性早熟模型，并于出生后第15天给予福幼方。观察阴道开口时间、卵巢和子宫湿重、血清激素水平及下丘脑相关基因的表达。我们发现，福幼方可延迟阴道开口，降低卵巢和子宫的湿重及系数，降低血清激素（雌二醇、卵泡刺激素和黄体生成素）水平及细胞GnRH水平，并下调下丘脑Kiss1、GPR54和GnRH的基因表达以及GT1-7细胞中GPR54和GnRH的基因和蛋白表达。综上所述，福幼方可能通过GPR54/GnRH信号通路缓解性早熟。
