Aghajanpour Fakhroddin, Soltani Reza, Afshar Azar, Abbaszadeh Hojjat-Allah, Fadaei Fathabadi Fatemeh, Moeinian Nafiseh, Aliaghaei Abbas, Dehghani Nejad Ali, Mastery Farahani Reza, Norouzian Mohsen, Abdollahifar Mohammad-Amin
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Student Research Committee, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Int J Reprod Biomed. 2024 Feb 23;22(1):17-30. doi: 10.18502/ijrm.v22i1.15238. eCollection 2024 Jan.
An increase in the temperature of the testis is associated with damage to the epithelium of seminiferous tubules and disruption of sperm production.
The current study aimed to investigate the effect of the Sertoli cell-conditioned medium (SCCM) on the blood-testis-barrier associated genes and spermatogenesis process following scrotal hyperthermia.
In this experimental study, 40 adult NMRI mice (8 wk, 25-30 gr) were allocated into 4 groups: I) control, II) DMEM (10 μl Dulbecco's Modified Eagle Medium), III) scrotal hyperthermia, and IV) scrotal hyperthermia+SCCM (10 μl SCCM). Hyperthermia was induced by placing the mice scrotum in water at 43 C for 20 min every other day for 10 days. Mice were treated every other day for 5 wk. Then the animals were euthanized, and the tails of epididymis were removed to analyze sperm parameters, testis were taken for stereological assessment, reactive oxygen spices and glutathione levels, and the expression of , , , and .
The results of sperm analysis indicated that SCCM-treated mice significantly increased sperm count and motility and reduced DNA fragmentation. In addition, histological and molecular findings showed that the volume of testicular tissue, the number of germ cells, the glutathione level, and the expression of , , , and genes were significantly increased in the SCCM-treated mice.
Findings suggest that growth factors of SCCM stimulate the proliferation and differentiation of germ cells through paracrine effects and upregulate the blood-testis-barrier-associated genes in mice subjected to scrotal hyperthermia.
睾丸温度升高与曲细精管上皮损伤及精子生成中断有关。
本研究旨在探讨支持细胞条件培养基（SCCM）对阴囊高温后血睾屏障相关基因及精子发生过程的影响。
在本实验研究中，将40只成年NMRI小鼠（8周龄，体重25 - 30克）分为4组：I）对照组，II）DMEM（10微升杜氏改良 Eagle培养基）组，III）阴囊高温组，IV）阴囊高温 + SCCM（10微升SCCM）组。通过每隔一天将小鼠阴囊置于43℃水中20分钟，持续10天来诱导高温。小鼠每隔一天接受治疗，持续5周。然后对动物实施安乐死，取出附睾尾部以分析精子参数，取睾丸进行体视学评估、检测活性氧和谷胱甘肽水平，以及检测 、 、 和 的表达。
精子分析结果表明，经SCCM处理的小鼠精子数量和活力显著增加，DNA碎片化减少。此外，组织学和分子学研究结果显示，经SCCM处理的小鼠睾丸组织体积、生殖细胞数量、谷胱甘肽水平以及 、 、 和 基因的表达均显著增加。
研究结果表明，SCCM的生长因子通过旁分泌作用刺激生殖细胞的增殖和分化，并上调阴囊高温小鼠血睾屏障相关基因的表达。
