Luo Chao, Lu Zhiying, Chen Yongli, Chen Xiaozhen, Liu Na, Chen Jing, Dong Shanwu
Department of Pediatrics, Wuhan Fourth Hospital, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430034, P.R. China.
Department of Pediatrics, Kunming Medical University Affiliated Kunming Children's Hospital, Kunming, Yunnan 650034, P.R. China.
Oncol Lett. 2021 Feb;21(2):161. doi: 10.3892/ol.2020.12422. Epub 2020 Dec 31.
The effects of microRNAs (miRNAs/miRs) on glioblastoma have attracted the attention of researchers in the last 7 years. However, the role of miR-640 and its targeted gene, Slit guidance ligand 1 (SLIT1), in the development of glioblastoma are not yet fully understood. The present study aimed to investigate the role of miR-640 in the proliferation and adhesion of glioblastoma. Reverse transcription-quantitative PCR analysis was performed to detect miR-640 and SLIT1 expression in glioblastoma tissues and cells. In addition, the Dual-luciferase reporter and RNA-pull down assays were performed to assess the association between miR-640 and SLIT1. The Cell Counting Kit-8, BrdU ELISA, cell adhesion and caspase-3 activity assays were also performed to assess cell viability, proliferation, adhesion and apoptosis of glioblastoma cells, respectively. The results demonstrated that miR-640 expression was upregulated in glioblastoma tissues and cells. In addition, miR-640 promoted the cell viability, proliferation and adhesion of glioblastoma cells, while inhibiting cell apoptosis. SLIT1, a direct downstream target of miR-640, was demonstrated to be downregulated in glioblastoma tissues and cells. Furthermore, overexpression of SLIT1 attenuated the promotive effect of miR-640 on glioblastoma cells. Taken together, these results suggest that miR-640 accelerates the proliferation and adhesion of glioblastoma cell lines by targeting and suppressing SLIT1.
在过去7年里，微小RNA（miRNA/miR）对胶质母细胞瘤的影响已引起研究人员的关注。然而，miR-640及其靶向基因Slit引导配体1（SLIT1）在胶质母细胞瘤发生发展中的作用尚未完全明确。本研究旨在探讨miR-640在胶质母细胞瘤增殖和黏附中的作用。采用逆转录定量PCR分析检测胶质母细胞瘤组织和细胞中miR-640和SLIT1的表达。此外，进行双荧光素酶报告基因检测和RNA下拉实验以评估miR-640与SLIT1之间的关系。还进行了细胞计数试剂盒-8、BrdU ELISA、细胞黏附及caspase-3活性检测，分别评估胶质母细胞瘤细胞的活力、增殖、黏附和凋亡情况。结果表明，miR-640在胶质母细胞瘤组织和细胞中表达上调。此外，miR-640促进胶质母细胞瘤细胞的活力、增殖和黏附，同时抑制细胞凋亡。SLIT1作为miR-640的直接下游靶点，在胶质母细胞瘤组织和细胞中表达下调。此外，SLIT1过表达减弱了miR-640对胶质母细胞瘤细胞的促进作用。综上所述，这些结果表明miR-640通过靶向抑制SLIT1来加速胶质母细胞瘤细胞系的增殖和黏附。
