Zhai Fengyu, Chen Xinfeng, He Qianyi, Zhang Heng, Hu Yongqiang, Wang Dan, Liu Shasha, Zhang Yi
Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.
Department of Radiotherapy, Puyang Oil Field General Hospital, Puyang, Henan 457000, P.R. China.
Oncol Lett. 2019 Aug;18(2):1922-1930. doi: 10.3892/ol.2019.10480. Epub 2019 Jun 14.
MicroRNAs (miRNAs/miRs), including miR-181, are closely linked to the development and progression of glioblastoma. However, the function of miR-181 in glioblastoma has not been fully clarified. The aim of the present study was to investigate the role of miR-181 in glioblastoma. miR-181 was revealed to be downregulated in glioblastoma tissues and cell lines, and associated with poor prognosis in patients with glioblastoma. Overexpression of miR-181 inhibited glioblastoma cell proliferation, invasion and migration, arrested glioblastoma cell cycle in the G1 phase and induced glioblastoma cell apoptosis. miR-181 was demonstrated to decrease expression of C-C motif chemokine ligand 8 (CCL8) by directly interacting with its 3'-untranslated region. Overexpression of CCL8 inversely reversed the proliferation, invasion and migration-promoting effects of miR-181 in glioblastoma cells. Furthermore, CCL8 was upregulated in glioblastoma tissues and was negatively correlated with miR-181 expression. These results indicate that miR-181 is a potential molecular biomarker or therapeutic target in the clinical management of glioblastoma.
包括miR-181在内的微小RNA（miRNA/miR）与胶质母细胞瘤的发生发展密切相关。然而，miR-181在胶质母细胞瘤中的功能尚未完全阐明。本研究的目的是探讨miR-181在胶质母细胞瘤中的作用。研究发现，miR-181在胶质母细胞瘤组织和细胞系中表达下调，且与胶质母细胞瘤患者的不良预后相关。miR-181的过表达抑制了胶质母细胞瘤细胞的增殖、侵袭和迁移，使胶质母细胞瘤细胞周期停滞于G1期并诱导细胞凋亡。研究表明，miR-181通过直接与其3'-非翻译区相互作用来降低C-C基序趋化因子配体8（CCL8）的表达。CCL8的过表达可反向逆转miR-181对胶质母细胞瘤细胞增殖、侵袭和迁移的促进作用。此外，CCL8在胶质母细胞瘤组织中上调，且与miR-181表达呈负相关。这些结果表明，miR-181是胶质母细胞瘤临床治疗中潜在的分子生物标志物或治疗靶点。
