Huber François, Lang Hans Peter, Lang Daniela, Wüthrich Daniel, Hinić Vladimira, Gerber Christoph, Egli Adrian, Meyer Ernst
Swiss Nanoscience Institute (SNI) Department of Physics University of Basel Klingelbergstrasse 82 Basel CH-4056 Switzerland.
Clinical Bacteriology and Mycology, University Hospital Basel Applied Microbiology Research Department of Biomedicine University of Basel Petersgraben 4 Basel 4031 Switzerland.
Glob Chall. 2020 Nov 30;5(2):2000066. doi: 10.1002/gch2.202000066. eCollection 2021 Feb.
The worldwide emergence of multidrug-resistant (MDR) bacteria is associated with significant morbidity, mortality, and healthcare costs. Rapid and accurate diagnostic methods to detect antibiotic resistance are critical for antibiotic stewardship and infection control measurements. Here a cantilever nanosensor-based diagnostic assay is shown to detect single nucleotide polymorphisms (SNPs) and genes associated with antibiotic resistance in Gram negative () and positive () bacteria, representing frequent causes for MDR infections. Highly specific RNA capture probes for SNPs ( or ) or resistance genes (, , and ) allow to detect the binding of bacterial RNA within less than 5 min. Serial dilutions of bacterial RNA indicate an unprecedented sensitivity of 10 fg µL total RNA corresponding to less than ten bacterial cells for SNPs and 1 fg µL total RNA for detection equivalent to single bacterial cell sensitivity.
多重耐药（MDR）细菌在全球范围内的出现与显著的发病率、死亡率及医疗成本相关。用于检测抗生素耐药性的快速准确诊断方法对抗生素管理和感染控制措施至关重要。在此展示了一种基于悬臂纳米传感器的诊断检测方法，可检测革兰氏阴性（）和阳性（）细菌中与抗生素耐药性相关的单核苷酸多态性（SNP）和基因，这些细菌是MDR感染的常见病因。针对SNP（或）或耐药基因（、和）的高度特异性RNA捕获探针能够在不到5分钟内检测到细菌RNA的结合。细菌RNA的系列稀释表明，对于SNP，前所未有的灵敏度为10 fg/µL总RNA，相当于不到十个细菌细胞；对于检测，灵敏度为1 fg/µL总RNA，等同于单个细菌细胞的灵敏度。
