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合成聚合物作为无动物源材料用于在人间充质干细胞培养中稳定碱性成纤维细胞生长因子

Synthetic polymers as xeno-free materials for stabilizing basic fibroblast growth factor in human mesenchymal stem cell cultures.

作者信息

Masuzawa Yoko, Kitazawa Manabu

机构信息

Institute for Innovation, Ajinomoto Co. Inc, 1-1 Suzuki-Cho, Kawasaki-Ku, Kawasaki City, Kanagawa, Japan.

出版信息

Biochem Biophys Rep. 2021 Jan 29;25:100929. doi: 10.1016/j.bbrep.2021.100929. eCollection 2021 Mar.

DOI:10.1016/j.bbrep.2021.100929
PMID:33553689
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7848777/
Abstract

Series of sulfonated polymers were evaluated as additives in cell culture media. Some of the compounds, such as sulfated polyvinyl alcohol (PVA), prevented denaturation and loss of basic fibroblast growth factor during cell culture and enhanced human mesenchymal stem cell proliferation. These compounds are xeno-free alternatives of heparin, an animal-derived sulfated saccharide, often used as an additive. To the best our knowledge, this study is the first to show that chemically defined synthetic chemicals, such as sulfated polyvinyl alcohol, can be used for this purpose.

摘要

对一系列磺化聚合物作为细胞培养基添加剂进行了评估。其中一些化合物，如硫酸化聚乙烯醇（PVA），可防止细胞培养过程中碱性成纤维细胞生长因子的变性和损失，并增强人间充质干细胞的增殖。这些化合物是肝素的无动物源替代品，肝素是一种常用作添加剂的动物源性硫酸化糖类。据我们所知，本研究首次表明化学定义的合成化学品，如硫酸化聚乙烯醇，可用于此目的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/463e/7848777/74a2eb8f876d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/463e/7848777/cb261a34918d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/463e/7848777/aa8ede2c7716/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/463e/7848777/466c38391555/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/463e/7848777/74a2eb8f876d/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/463e/7848777/cb261a34918d/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/463e/7848777/aa8ede2c7716/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/463e/7848777/466c38391555/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/463e/7848777/74a2eb8f876d/gr4.jpg

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Bioconjug Chem. 2019 Mar 20;30(3):833-840. doi: 10.1021/acs.bioconjchem.8b00921. Epub 2019 Feb 8.
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Effects of Human Full-length Amelogenin and C-terminal Amelogenin Peptide on the Proliferation of Human Mesenchymal Stem Cells Derived from Adipose Tissue.
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Human and Rat Bone Marrow-Derived Mesenchymal Stem Cells Differ in Their Response to Fibroblast Growth Factor and Platelet-Derived Growth Factor.人骨髓间充质干细胞和大鼠骨髓间充质干细胞对成纤维细胞生长因子和血小板衍生生长因子的反应不同。
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