Azeem Muhammad, Qaisar Rizwan, Karim Asima, Ranade Anu, Elmoselhi Adel
Department of Applied Physics and Astronomy, College of Sciences, University of Sharjah, 27272, Sharjah, United Arab Emirates.
Department of Basic Medical Sciences, College of Medicine, University of Sharjah, 27272, Sharjah, United Arab Emirates.
Biochem Biophys Rep. 2021 Jan 29;25:100930. doi: 10.1016/j.bbrep.2021.100930. eCollection 2021 Mar.
Hind-limb unloaded (HU) mouse is a well-recognized model of muscle atrophy; however, the molecular changes in the skeletal muscle during unloading are poorly characterized. We have used Raman spectroscopy to evaluate the structure and behavior of signature molecules involved in regulating muscle structural and functional health. The Raman spectroscopic analysis of gastrocnemius muscles was compared between 16-18 weeks old HU c57Bl/6J mice and ground-based controls. The spectra showed that the signals for asparagine and glutamine were reduced in HU mice, possibly indicating increased catabolism. The peaks for hydroxyproline and proline were split, pointing towards molecular breakdown and reduced tendon repair. We also report a consistently increased intensity in> 1300 cm range in the Raman spectra along with a shift towards higher frequencies in the HU mice, indicating activation of sarcoplasmic reticulum (SR) stress during HU.
后肢去负荷（HU）小鼠是一种公认的肌肉萎缩模型；然而，在去负荷过程中骨骼肌的分子变化特征尚不明确。我们使用拉曼光谱来评估参与调节肌肉结构和功能健康的标志性分子的结构和行为。对16 - 18周龄的HU c57Bl/6J小鼠和地面饲养的对照小鼠的腓肠肌进行了拉曼光谱分析。光谱显示，HU小鼠中天冬酰胺和谷氨酰胺的信号降低，这可能表明分解代谢增加。羟脯氨酸和脯氨酸的峰出现分裂，表明分子分解和肌腱修复能力下降。我们还报告，在HU小鼠中，拉曼光谱在> 1300 cm范围内的强度持续增加，同时向更高频率偏移，表明在HU过程中肌浆网（SR）应激被激活。
