Xu Xiaoxin, Wang Zhongxing, Guo Lingling, Xu Xinxin, Wu Aihong, Kuang Hua, Sun Li, Song Shanshan, Xu Chuanlai
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Republic of China.
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi 214122, People's Republic of China.
ACS Omega. 2021 Jan 18;6(4):2559-2569. doi: 10.1021/acsomega.0c04422. eCollection 2021 Feb 2.
Based on the successful derivation of a hapten, we prepared and optimized a murine monoclonal antibody against imidocarb, with an IC of 2.22 ng/mL and a limit of detection (LOD) of 0.45 ng/mL. Cross-experiment results showed that the cross-over rate for 4,4'-dinitrocarbanilide was 18.12%, and the cross-reactivity with other analogues when using the ic ELISA was less than 0.1%. We used the developed ic-ELISA to detect the addition and recovery of imidocarb in milk and beef samples, and values were 86.0-93.5 and 84.5-101.2%, respectively. The preparation of an immunochromatographic test strip based on gold nanoparticles was used for the rapid identification of imidocarb in milk and beef samples. When assessed by the naked eye, the visual LOD for imidocarb in milk and beef samples was 5 and 10 ng/mL, and the cut-off values were 20 and 50 ng/mL, respectively. Because of its high sensitivity, specificity, and simplicity, the test strip can be used for on-site testing and rapid screening of imidocarb in food samples.
基于成功衍生出一种半抗原，我们制备并优化了一种抗咪唑苯脲的鼠单克隆抗体，其半数抑制浓度（IC）为2.22 ng/mL，检测限（LOD）为0.45 ng/mL。交叉实验结果表明，4,4'-二硝基碳酰苯胺的交叉率为18.12%，使用间接竞争酶联免疫吸附测定（ic ELISA）时与其他类似物的交叉反应率小于0.1%。我们使用所开发的ic ELISA检测牛奶和牛肉样品中咪唑苯脲的添加量和回收率，其值分别为86.0 - 93.5%和84.5 - 101.2%。基于金纳米颗粒制备免疫层析试纸条用于快速鉴定牛奶和牛肉样品中的咪唑苯脲。通过肉眼评估时，牛奶和牛肉样品中咪唑苯脲的视觉检测限分别为5和10 ng/mL，临界值分别为20和50 ng/mL。由于其高灵敏度、特异性和简便性，该试纸条可用于食品样品中咪唑苯脲的现场检测和快速筛查。
