Department of Neuroscience, University of Kentucky College of Medicine, Lexington, KY 40536-0298, USA.
Markey Cancer Center, Lexington, KY 40536-0298, USA.
STAR Protoc. 2021 Jan 29;2(1):100304. doi: 10.1016/j.xpro.2021.100304. eCollection 2021 Mar 19.
N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020).
N-糖链和脂质是在细胞过程中发挥重要作用的结构代谢物。它们在组织中都呈现出独特的区域分布；因此，对这些代谢物的空间分析对于我们理解细胞生理学至关重要。基质辅助激光解吸/电离 - 质谱成像（MALDI-MSI）是一种创新技术，可实现具有空间分布的分析物的检测。本工作流程详细介绍了一种 MALDI-MSI 协议，用于在应用酶和 MALDI 基质后从组织中对 N-糖链和脂质进行空间分析。有关此协议的使用和执行的完整详细信息，请参阅 Drake 等人（2018 年）和 Andres 等人（2020 年）。
