基于基质辅助激光解吸电离飞行时间质谱（MALDI-2-TOF-MS）的负离子模式N-聚糖质谱成像
Negative Ion-Mode N-Glycan Mass Spectrometry Imaging by MALDI-2-TOF-MS.
作者信息
Soltwisch Jens, Heijs Bram
机构信息
Center for Proteomics & Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Biomedical Mass Spectrometry, Institute of Hygiene, University Hospital Munster, Münster, Germany.
出版信息
Methods Mol Biol. 2023;2688:173-186. doi: 10.1007/978-1-0716-3319-9_15.
Matrix-assisted laser desorption/ionization mass spectrometry imaging with laser-induced postionization (MALDI-2-MSI) has proven a powerful tool for the in situ analysis of N-linked glycosylation, or N-glycans, directly from clinical tissue samples. Here we describe a sample preparation protocol for the analysis of N-glycans from formalin-fixed, paraffin-embedded tissue sections.
基质辅助激光解吸/电离质谱成像结合激光诱导后电离（MALDI-2-MSI）已被证明是一种直接从临床组织样本原位分析N-连接糖基化或N-聚糖的强大工具。在此，我们描述一种用于分析福尔马林固定、石蜡包埋组织切片中N-聚糖的样本制备方案。
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