Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan.
STAR Protoc. 2021 Feb 1;2(1):100314. doi: 10.1016/j.xpro.2021.100314. eCollection 2021 Mar 19.
Renal progenitor cells induced from pluripotent stem cells have attracted attention as a cell source for organ regeneration. Here, we report an protocol for the regeneration of urine-producing nephrons, i.e., neo-nephrons, in mice. We outline steps to transplant exogenous renal progenitor cells into the nephrogenic zone of transgenic mice and subsequently analyze these neo-nephrons. For complete details on the use and execution of this protocol, please refer to Fujimoto et al. (2020).
多能干细胞诱导的肾祖细胞作为器官再生的细胞来源引起了关注。在这里，我们报告了一种在小鼠中再生产生尿液的肾单位（即新肾单位）的方案。我们概述了将外源性肾祖细胞移植到转基因小鼠的肾发生区并随后分析这些新肾单位的步骤。有关该方案使用和实施的完整详细信息，请参见 Fujimoto 等人（2020 年）。
